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目的:比较西洋参冠瘿组织与西洋参药材人参总皂苷含量及各人参皂苷含量的差异,为采用生物技术方法生产人参皂苷提供理论依据。方法:采用香草醛-冰醋酸-高氯酸紫外显色法测定西洋参冠瘿组织与西洋参药材中总皂苷含量;用HPLC法测定西洋参冠瘿组织与西洋参药材中人参皂苷Rb1、Rb3、Rc、Re的含量。结果:(1)培养27d西洋参冠瘿组织总皂苷积累量达到最高,与西洋参药材总皂苷含量相当;(2)西洋参冠瘿组织中人参皂苷Rb1、Re的含量约为西洋参药材的50%,人参皂苷Rc的含量约为西洋参药材的80%,人参皂苷Rb3的含量远远高于西洋参药材,约为西洋参药材的16倍。结论:西洋参冠瘿组织可作为大规模生产人参皂苷的新途径。
Objective: To compare the content of ginsenosides and the content of ginsenosides in G. quinquefolium and American ginseng, and to provide a theoretical basis for the production of ginsenosides by biotechnology. Methods: The contents of total saponins in the crown cultivars of American ginseng and American ginseng were determined by UV-visible colorimetry with vanillin-glacial acetic acid-perchloric acid. The ginsenosides Rb1, Rb3, Rc, Re of American ginseng and Ginseng were determined by HPLC. The content. Results: (1) The total saponin accumulation in the crown of American ginseng cultured for 27 days was the highest, which was the same as the content of total saponins in American ginseng; (2) The content of ginsenosides Rb1 and Re in the tissues of American ginseng crown was about 50% of that of the American ginseng The content of saponin Rc is about 80% of that of American ginseng, and the content of ginsenoside Rb3 is much higher than that of American ginseng, about 16 times that of American ginseng. Conclusion: Western ginseng crown gall tissue can be used as a new way of large-scale production of ginsenosides.