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目的:研究3′-大豆苷元磺酸钠(DSS)对离体心脏缺血再灌注损伤的保护作用及NO、ANG Ⅱ和ANP含量的变化。方法:应用大鼠离体心肌缺血再灌注损伤模型,在灌流液中加入高、中、低剂量的DSS。测定冠脉流量、左心室收缩压及心肌组织NO、ANG Ⅱ和ANP含量的变化。结果:各剂量的3′-大豆苷元磺酸钠均能有效地增加冠脉流量,增强左心室收缩压;三种剂量的DSS均能升高心肌组织NO含量(P<0.05);高剂量的DSS使心肌组织ANG Ⅱ(P<0.05)含量降低,低剂量的DSS使ANP含量升高(P<0.05)。结论:DSS可通过增加NO、ANP的合成,减少ANG Ⅱ的释放,扩张冠脉,增强心肌收缩力,从而对离体心脏缺血再灌注损伤起保护作用。
OBJECTIVE: To investigate the protective effect of sodium 3′-daidzein sulfonate (DSS) on ischemic reperfusion injury and the changes of NO, ANG II and ANP content in isolated hearts. METHODS: Rat isolated myocardial ischemia-reperfusion injury model was used to add high, middle and low doses of DSS to the perfusate. Changes in coronary flow, left ventricular systolic pressure, and myocardial NO, ANG II, and ANP levels were measured. Results: Each dose of 3′-daidzein sodium sulfonate can effectively increase coronary blood flow and enhance left ventricular systolic pressure; three doses of DSS can increase NO content in myocardial tissue (P<0.05); DSS reduced myocardial ANG II (P<0.05), and low dose DSS increased ANP content (P<0.05). Conclusion: DSS can protect the isolated heart from ischemia-reperfusion injury by increasing the synthesis of NO and ANP, reducing the release of ANG II, expanding the coronary artery, and enhancing the myocardial contractility.