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目的探讨不同剂量的DEHP对子代大鼠学习记忆的影响。方法成熟Wistar大鼠,在妊娠期7～16 d,每天经口灌胃给予DEHP高(1000 mg/kg)、中(750 mg/kg)、低(250 mg/kg)三剂量组,对照组经口灌胃给予照物玉米油。采用跳台实验和避暗实验方法,观察DEHP对子代大鼠学习记忆障碍的影响。结果神经行为学实验方法结果显示,跳台实验中高、中、低剂量组与对照组比较,错误次数明显增多,平均潜伏期明显缩短(P<0.05);高剂量组与中、低剂量组比较,子代大鼠错误次数明显增多,潜伏期明显缩短(P<0.05)。避暗实验中高、中、低剂量组子代大鼠进入暗箱的潜伏期缩短明显低于对照组(P<0.05)及子代大鼠5 min内受电击次数明显高于对照组(P<0.05);高剂量组子代大鼠进入暗箱的潜伏期缩短与中、低剂量组比较有统计学意义(P<0.05);高剂量组子代大鼠进入暗室的错误次数增多与低剂量组比较有显著性(P<0.05)。结论 DEHP对子代大鼠中枢神经系统损害有一定的影响,子代大鼠学习记忆能力明显减退。
Objective To investigate the effects of different doses of DEHP on learning and memory in offspring rats. Methods The mature Wistar rats were orally administered with three doses of DEHP (1000 mg / kg), medium (750 mg / kg) and low (250 mg / kg) Oral gavage given corn oil. The effects of DEHP on learning and memory deficits in offspring rats were observed by using the platform jumping test and avoidance experiment. Results The neurobehavioral test results showed that the number of errors was significantly increased and the average latency was significantly shortened (P <0.05) in the high, middle and low dose groups compared with the control group in the jumping test. Compared with the medium and low dose groups, The number of rats in rats increased significantly, and latency was significantly shorter (P <0.05). In the dark-avoidance experiment, the latency of entering the dark box in the offspring rats in the high, medium and low dose groups was significantly shorter than that in the control group (P <0.05) and in the offspring rats in 5 minutes was significantly higher than that in the control group (P <0.05) (P <0.05). In the high-dose group, the increase in the number of false-positive rats entering the darkroom was significantly lower than that in the low-dose group (P <0.05). Conclusion DEHP has a certain influence on the damage of central nervous system in offspring rats, and the learning and memory abilities of offspring rats are obviously decreased.