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目的探讨雌激素对小鼠肝脏纤维化的保护作用。方法 SPF级雄性C57BL/6J小鼠15只随机均分为三组:橄榄油组(Oil组),四氯化碳(CCl4)处理组(CCl4组),CCl4处理加雌激素1mg/kg干预组(CCl4+E组)。1周后用ELISA法检测血清ALT水平;HE染色、马松三色法和猩红染色法检测肝脏纤维化程度;Western blot检测肝脏组织Ⅰ型胶原蛋白表达,qRT-PCR检测Ⅰ型胶原α1链(Col1α1)、Ⅰ型胶原α2链(Col1α2)和α-平滑肌肌动蛋白(α-SMA)基因表达和免疫荧光染色检测α-SMA荧光表达强度。结果与CCl4组比较,CCl4+E组能降低小鼠ALT水平(P<0.05),CCl4+E组肝脏Col1α1、Col1α2和α-SMA的基因表达均较CCl4组降低(均P<0.05)。在肝星状细胞(HSC)-T6细胞系中,胶原基因启动子转录活性增加,雌激素能剂量依懒性下调胶原基因启动子的激活能力(P<0.05)。结论雌激素对CCl4诱导的小鼠肝脏纤维化具有保护作用,主要是通过抑制HSC细胞活化、降低细胞外基质的合成延缓肝纤维化进程。
Objective To investigate the protective effect of estrogen on liver fibrosis in mice. Methods Fifteen SPF male C57BL / 6J mice were randomly divided into three groups: Oil group, CCl4 group, CCl4 plus estrogen 1 mg / kg group (CCl4 + E group). The level of serum ALT was detected by ELISA after 1 week. The degree of liver fibrosis was detected by HE staining, Masson’s trichrome and scarlet staining. The expression of type Ⅰ collagen in liver tissue was detected by Western blot. Col1α1, Col1α2, and α-SMA, and the intensity of α-SMA expression was detected by immunofluorescence staining. Results Compared with CCl4 group, CCl4 + E group decreased ALT level in mice (P <0.05). The gene expressions of Col1α1, Col1α2 and α-SMA in CCl4 + E group were lower than those in CCl4 group (all P <0.05). In hepatic stellate cells (HSC) -T6 cell line, the transcriptional activity of collagen gene promoter increased, estrogen dose dependently decreased the activation of collagen gene promoter (P <0.05). Conclusion Estrogen has a protective effect on hepatic fibrosis induced by CCl4 in mice, mainly through inhibiting the activation of HSC cells and reducing the synthesis of extracellular matrix to delay the progression of hepatic fibrosis.