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目的 从不同剂量氟化钠 (NaF)对大鼠成骨细胞 (OB)功能表达的影响 ,研究氟剂促进成骨作用的机制及剂量效应关系。方法 经酶消化法从新生 2 4hSpregne Dawley (SD)种乳鼠头盖骨分离得到的成骨细胞。在 10 -7～ 5× 10 -4mol/LNaF中培养。用生化法测定细胞碱性磷酸酶 (ALP)活性 ,用放射免疫法测定培养液中骨钙素 (OC)含量。结果 NaF对细胞ALP活性的影响呈双向 ,即低浓度NaF(10 -7～ 10 -5mol/L)增加ALP活性 ,高浓度NaF (10 -4～ 5× 10 -4mol/L)则抑制ALP活性 ;对骨钙素产生和分泌的影响则呈单向 ,即各浓度NaF均刺激细胞骨钙素分泌 ,并与氟剂剂量呈正相关 ,其中 5× 10 -4mol/L组骨钙素水平最高 ,与对照组相比增加 6 7 14 % (P <0 0 5 )。结论 适量氟剂能增加成骨细胞ALP的活性及骨钙素的产生 ,促进成骨细胞的骨形成功能 ,但有效剂量范围狭窄
OBJECTIVE: To study the effect of different doses of sodium fluoride (NaF) on osteoblasts (OB) expression in rats and to explore the mechanism and mechanism of the effect of fluoride agents on osteogenesis. METHODS: Osteoblasts isolated from the skull of neonatal 24 h Sprague Dawley (SD) neonates were digested by enzyme. Cultured at 10 -7 to 5 × 10 -4 mol / L NaF. The activity of alkaline phosphatase (ALP) was measured by biochemical method, and the content of osteocalcin (OC) in culture solution was determined by radioimmunoassay. Results The effect of NaF on the ALP activity of the cells was bi-directional, that is, the low concentration of NaF (10 -7 ~ 10 -5 mol / L) increased ALP activity while the high concentration of NaF (10 -4 ~ 5 × 10 -4 mol / L) ; The effect on osteocalcin production and secretion was unidirectional, that is, each concentration of NaF stimulated the secretion of osteocalcin, and was positively correlated with the dose of fluorine agent, of which 5 × 10 -4 mol / L group had the highest level of osteocalcin, Compared with the control group, an increase of 6 7 14% (P 0 05). CONCLUSION: The appropriate amount of Fluoride can increase the activity of ALP and the production of osteocalcin in osteoblasts and promote the bone formation of osteoblasts, but the effective dose range is narrow