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目的 体外培养、分离、纯化P2期小鼠视神经胶质细胞,并观察其生物学特性。方法 纤颅开眶获取P2期小鼠管内段视神经2mm,组织块法培养,振荡法分离纯化,免疫组织化学方法鉴定,流式细胞仪检测纯度。结果 视神经星形胶质细胞体外迅速分裂增殖,少突胶质细胞生长缓慢,传代困难,振荡法分离纯化的细胞纯度高,星形胶质细胞GFAP染色阳性,阳性率96.2％,少突胶质细胞MBP染色阳性,阳性率96％。结论 组织块法培养、振荡法分离纯化P2期小鼠视神经星形和少突胶质细胞纯度高,是进一步研究视神经胶质细胞的结构和功能的叶靠材料。
Objective To culture, isolate and purify optic nerve glial cells of P2 mice in vitro and observe its biological characteristics. Methods The optic nerve of the P2 segment of the optic canal in P2 period mice was obtained by intracranial approach and cultured by tissue block method. The isolated and purified Oscillatory method was identified by immunohistochemical method. The purity was detected by flow cytometry. Results The optic nerve astrocytes rapidly divided and proliferated in vitro. The oligodendrocytes grew slowly and were difficult to passage. The purity of astrocytes was high, the positive rate of astrocyte GFAP was 96.2%, oligodendrocyte Cell MBP staining positive, the positive rate of 96%. Conclusions Tissue culture and oscillatory method for the purification and purification of the optic nerve in P2 phase mice and the high purity of oligodendrocytes are the materials for the further study of the structure and function of optic nerve glial cells.