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目的检测储存前滤除白细胞对红细胞悬液中致热源性细胞因子含量的影响,以及致热源性细胞因子与非溶血性发热性输血反应(FNHTR)的关系,以期得到较为全面的认识。方法随机抽取普通红细胞悬液及去除白细胞的红细胞悬液2种成分;每种成分按照自制备到输注前的储存时间分组;用ELISA方法检测致热源性细胞因子含量,观察输血反应情况。结果 3种致热源性细胞因子含量在检测期内的不同时间点略有升高,与储存时间无平行关系。2例发生发热性输血反应病例中致热源性细胞因子含量均非最高值。结论红细胞悬液因为储存在较低温度下,所以在储存过程中致热源性细胞因子释放量较小,不足以引起非溶血性发热反应。
Objective To investigate the effect of leukocyte depletion on the production of cytokines in erythrocytes suspension before storage and the relationship between cytokines and non-hemolytic transfusion reaction (FNHTR) in order to obtain a more comprehensive understanding. Methods Two components of common erythrocyte suspension and erythrocyte suspension of leukocyte were randomly selected; each component was divided into groups according to the storage time from preparation to infusion; the content of cytokines induced by heat was detected by ELISA, and the transfusion reaction was observed. Results The contents of three cytokines induced by heat were slightly increased at different time points during the test period, but not in parallel with the storage time. Two cases of fever caused by transfusion reactions induced pyrogen cytokine levels were not the highest. Conclusion Because of the lower temperature stored in the erythrocyte suspension, the amount of cytokines released during storage is not enough to cause nonhemolytic fever.