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目的探讨肺炎支原体(MP)荧光定量PCR检测在儿童肺炎支原体肺炎(MPP)中的临床应用价值。方法选择支原体肺炎患儿108例,分别于入院当天、治疗后7d、14d、21d留取呼吸道分泌物(咽拭子或鼻咽拭子)两份,一份采用MP荧光定量PCR法检测其咽试子标本中MP DNA水平,另一份用特异性的MP快速液体培养基进行培养。结果肺炎支原体感染早期MP培养与MP-DNA阳性率分别为86.11%和89.81%,差异无统计学意义(P>0.05),两种方法的符合率为95.4%。用药后,MP培养的阳性率明显降低。结论荧光定量PCR动态监测肺炎支原体病原体含量,可作为MP早期诊断和疗效观察的良好指标,作为治愈停药的可靠指标。
Objective To investigate the clinical value of Mycoplasma pneumoniae (MP) quantitative PCR in children with Mycoplasma pneumoniae pneumonia (MPP). Methods A total of 108 children with mycoplasmal pneumonia were selected. Two respiratory secretions (throat swab or nasopharyngeal swab) were collected on the day of admission, 7d, 14d and 21d after treatment, respectively. One of them was detected by MP fluorescence quantitative PCR The level of MP DNA in the test specimen was tested, and the other was incubated with a specific MP fast liquid medium. Results The positive rate of MP-DNA and MP-DNA in early stage of Mycoplasma pneumoniae infection were 86.11% and 89.81% respectively, with no significant difference (P> 0.05). The coincidence rate of the two methods was 95.4%. After treatment, the positive rate of MP culture decreased significantly. Conclusion The dynamic detection of Mycoplasma pneumoniae pathogen by real-time fluorescence quantitative PCR can be used as a good indicator of early diagnosis and curative effect observation of MP as a reliable indicator of cure withdrawal.