目的　观察EDRF1基因在HEL细胞中对红细胞终末分化和珠蛋白表达的扮演的角色。方法　构建EDRF1真核正义和反义表达载体 ,转染HEL细胞并筛选稳定表达细胞克隆。然后利用Northernblot和反转录PCR (reversetranscription polymerasechainreaction ,RT PCR)的方法观察红系标志基因的表达水平改变。凝胶阻滞电泳 (electrophoresismobilityshiftassay ,EMSA)的方法观察红系转录因子的DNA结合活性的改变。结果　与对照相比 ,EDRF1过表达的HEL细胞中α 珠蛋白合成明显增加 ,EDRF1反义表达载体转染的HEL细胞中的α、γ 珠蛋白合成下调 ,而红细胞生成素受体 (erythropoietinreceptor ,ER)表达水平没有明显改变 ,这提示EDRF1并非通过调控ER信号通路而影响HEL细胞的红细胞分化。红细胞特异的转录因子GATA 1和NF E2mRNA的表达在转染前后没有明显改变。另外 ,GATA 1转录因子的DNA结合活性在反义表达载体转染的HEL细胞中受到明显的抑制 ,而NF E2的转录活性则没有明显的改变。结论　EDRF1下调珠蛋白的合成是通过抑制红系特异的转录因子GATA 1的DNA结合活性实现的 Objective To investigate the role of EDRF1 gene in terminal cell differentiation and globin expression in HEL cells. Methods The eukaryotic sense and antisense eukaryotic expression vectors of EDRF1 were constructed and transfected into HEL cells and the stable cell clones were selected. Then, the expression of erythroid marker gene was observed by Northern blot and reverse transcriptase polymerase chain reaction (RT PCR). Gel electrophoresis (electrophoresisismobilityshiftassay, EMSA) method to observe the erythroid transcription factor DNA binding activity changes. Results Compared with the control, α-globin synthesis was significantly increased in ED cells overexpressing EDRF1 and α, γ globin synthesis was down-regulated in HEL cells transfected with the EDRF1 antisense expression vector, whereas erythropoietin receptor (ER ) Expression did not change significantly, suggesting that EDRF1 does not affect the red blood cell differentiation of HEL cells by regulating the ER signaling pathway. The expression of erythrocyte-specific transcription factors GATA 1 and NF E2 mRNA did not change significantly before and after transfection. In addition, the DNA binding activity of GATA 1 transcription factor was significantly inhibited in HEL cells transfected with antisense expression vector, while the transcription activity of NF E2 was not significantly changed. Conclusion The synthesis of downregulation of globin by EDRF1 is achieved by inhibiting the DNA-binding activity of erythroid-specific transcription factor GATA 1