通过单因素试验选取试验因素与水平,再根据Box-Behnken的中心组合试验设计及响应面法分析建立二次回归模型,对提取温度、提取时间和料液比进行优化组合,研究了野生黑皮鸡枞菌多糖的提取工艺。结果表明,黑皮鸡枞菌多糖提取的最佳工艺条件为:提取温度82℃、提取时间3.9 h、水料比26︰1(m L/g)。在此工艺条件下,野生黑皮鸡枞菌多糖得率为26.72%。 The factors and levels of test were selected by single factor test. Based on Box-Behnken’s central combination design and response surface analysis, a quadratic regression model was established to optimize the extraction temperature, extraction time and the ratio of solid to liquid. Extraction of Polysaccharide from Termitomyces. The results showed that the optimal extraction conditions were: extraction temperature 82 ℃, extraction time 3.9 h, ratio of water to material 26:1 (m L / g). Under these conditions, the yield of wild black-bone Termitomyces polysaccharide was 26.72%.