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目的 探索眼局部应用榄香烯对角膜碱烧伤诱发新生血管的抑制作用 ,以期为榄香烯抗新生血管发生的临床应用提供依据。方法 采用 0 .5mol·L-1氢氧化钠烧伤兔眼角膜诱发新生血管模型。研究分为 2组 :组Ⅰ ,于碱烧伤后第 2天每日结膜下注射榄香烯 2 5mg,连续 5d ,1周后重复给药 ,连续 5d。组Ⅱ ,采用 5 FU结膜下注射 12 .5mg ,给药方法与组Ⅰ同。同时 ,配以非给药造模对照组。应用裂隙灯检查、角膜照相、眼计算机立体分析系统 ,观察并分析新生血管面积 ,每周 1次 ,连续 7周。比较两种药物对新生血管作用的动态变化。另外 ,正常兔、大鼠各 10只结膜下注射榄香烯和 5 FU ,观察毒性作用及耐受情况 ,以期找到安全剂量。结果 眼局部注射榄香烯的毒副作用小 ,重复给药动物反应耐受。有 2 0 %~ 30 %出现眼部刺激、红肿、畏光及角膜浸润。组Ⅰ、组Ⅱ和非给药造模对照组在 7周时角膜新生血管面积分别为 31 .88mm2 、4 9.84mm2 和 6 5.2 0mm2 ,组间比较有显著性统计学差异 (P <0 .0 5)。结论 本研究结果显示结膜下注射榄香烯和 5 FU对角膜碱烧伤诱发的新生血管形成有抑制作用 ,榄香烯作用优于 5 FU。榄香烯对已形成的角膜新生血管也有一定的消退作用。眼局部应用无明显的毒副作用 ,是安全有效的给
OBJECTIVE To explore the inhibitory effect of elemene on the neovascularization induced by corneal alkali burn in order to provide evidence for the clinical application of elemene against neovascularization. Methods Rabbit cornea was induced by 0.5 mol·L-1 sodium hydroxide to induce neovascularization. The study was divided into two groups: group Ⅰ, 2 mg of elemene was subconjunctivally injected on the second day after the alkali burn for 5 consecutive days, and repeated for 1 week after administration for 5 days. Group Ⅱ, using 5 FU subconjunctival injection of 12.5mg, administration method and group Ⅰ with. At the same time, with non-medication model control group. Slit lamp examination, corneal photography, eye computer three-dimensional analysis system, observation and analysis of neovascular area, once a week for 7 weeks. Compare the dynamic changes of the effects of two drugs on neovascularization. In addition, 10 rabbits and 10 rats were injected with elemene and 5 FU subconjunctivally to observe the toxic effects and tolerance, in order to find a safe dose. Results Eye injection of elemene with small side effects, repeated administration of animal response tolerance. There are 20% ~ 30% eye irritation, swelling, photophobia and corneal infiltration. The corneal neovascularization area of group Ⅰ, group Ⅱ and non-administration model control group were 31.88mm2, 48.84mm2 and 52.200mm2 respectively at 7 weeks, there was significant difference between the two groups (P <0. 0 5). Conclusions The results of this study showed that subconjunctival injection of elemene and 5 FU inhibited neovascularization induced by alkali burn of cornea. Elemene was superior to 5 FU. Elemene on the formation of corneal neovascularization also has some effect. Eye application of local no obvious side effects, is safe and effective to