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目的:探讨黄芪多糖(astragalus polysaccharide,APS)体外是否具有化疗增敏作用及其可能机制。方法:用MTT法检测APS协同化疗药对H22/ADM敏感性、用流式细胞术、免疫印迹法和荧光定量PCR进一步检测其P-糖蛋白(P-GP)功能、P-GP和mdr1mRNA表达。结果:在终浓度0.8～500μg.mL-1范围内APS可降低阿霉素(adriamycin,ADM)、5-氟脲嘧啶(5-fluorouracil,5-Fu)、顺铂(cisplatin,DDP)、依托泊苷(etoposide,VP-16)对H22/ADM的IC50值,使其Rho-123潴留增多,增加荧光强度值,下调P-GP和mdr1mRNA表达。结论:APS可增强H22/ADM化疗敏感性,机制可能与降低P-GP外排功能,下调P-GP和mdr1mRNA表达有关。
Objective: To investigate whether astragalus polysaccharide (APS) has chemosensitization effect in vitro and its possible mechanism. Methods: The sensitivity of APS and chemotherapeutic drugs to H22 / ADM was detected by MTT assay. The expression of P-GP and mdr1 mRNA were detected by flow cytometry, Western blotting and real-time PCR. . Results: APS could reduce adriamycin (ADM), 5-fluorouracil (5-Fu) and cisplatin (DDP) in the range of 0.8 ~ 500μg.mL- IC50 values of etoposide (VP-16) to H22 / ADM increased the retention of Rho-123, increased the fluorescence intensity and down-regulated the expression of P-GP and mdr1 mRNA. Conclusion: APS can enhance the chemosensitivity of H22 / ADM. The mechanism may be related to the decrease of P-GP efflux function and down-regulation of P-GP and mdr1 mRNA expression.