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目的:探讨间歇低氧暴露对高脂膳食肥胖大鼠胰岛素抵抗的影响。方法:40只雄性SD大鼠随机分为普通膳食常氧组、普通膳食间歇低氧组、高脂膳食常氧组、高脂膳食间歇低氧组。普通膳食采用普通饲料,高脂膳食采用高脂饲料,间歇低氧暴露采用常压低氧方法,氧浓度控制在15.4%,每天10小时。干预6周后取材测试大鼠内脏脂肪总量、空腹血糖和胰岛素,并计算胰岛素敏感指数。结果:与普通膳食常氧组相比,高脂膳食常氧组大鼠Lee指数、内脏脂肪总量和血清胰岛素水平极显著增加而胰岛素敏感指数极显著下降(P<0.01),但普通膳食间歇低氧组大鼠Lee指数、内脏脂肪总量显著减少(P<0.05,P<0.01),胰岛素敏感指数显著升高(P<0.05);与高脂膳食常氧组比较,高脂膳食间歇低氧组大鼠Lee指数、内脏脂肪总量和胰岛素水平极显著下降(P<0.01),胰岛素敏感指数极显著提高(P<0.01)。相关性分析显示,内脏脂肪总量与胰岛素水平存在一定正相关,且胰岛素水平和胰岛素敏感指数存在高度负相关(P<0.01)。结论:体内脂肪总量增加可使血清胰岛素水平升高,胰岛素敏感性下降,而间歇低氧暴露可以减少高脂膳食大鼠体内脂肪含量,提高胰岛素敏感性,改善胰岛素抵抗。
Objective: To investigate the effect of intermittent hypoxia exposure on insulin resistance in obese rats with high-fat diet. Methods: Forty male SD rats were randomly divided into normal diet normoxia group, normal diet intermittent hypoxia group, high fat diet normoxia group and high fat diet intermittent hypoxia group. Ordinary diets using ordinary feed, high-fat diet using high-fat diet, intermittent hypoxia exposure using atmospheric hypoxia method, oxygen concentration control at 15.4%, 10 hours a day. Six weeks after the intervention, the total amount of visceral fat, fasting blood glucose and insulin in rats were measured, and the insulin sensitivity index was calculated. Results: Compared with the normal dietary normoxia group, the Lee index, total visceral fat and serum insulin levels increased significantly and the insulin sensitivity index decreased significantly (P <0.01) In the hypoxia group, the Lee index, the total amount of visceral fat decreased significantly (P <0.05, P <0.01), and the insulin sensitivity index increased significantly (P <0.05). Compared with the normoxic group, In the oxygen group, the Lee index, visceral fat mass and insulin level decreased significantly (P <0.01), and the insulin sensitivity index increased significantly (P <0.01). Correlation analysis showed that there was a positive correlation between total visceral fat and insulin level, and there was a highly negative correlation between insulin level and insulin sensitivity index (P <0.01). Conclusion: The increase of total body fat can increase the serum insulin level and reduce the insulin sensitivity. Intermittent hypoxia exposure can reduce the body fat content, improve the insulin sensitivity and improve the insulin resistance in high fat diet rats.