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目的： Ｔｃ（Ⅴ） Ｄ Ｍ Ｓ能够对多种肿瘤进行显像，但显像机制尚无定论。本研究拟观察葡萄糖介导的酸中毒肿瘤摄取 Ｔｃ（Ⅴ） Ｄ Ｍ Ｓ程度的影响，以证实肿瘤摄取该药时对ｐ Ｈ 值的敏感性。方法：荷 Ｅｈｒｌｉｃｈ 腹水癌细胞株的小鼠 １５ 只，肿瘤种植部位为大腿内侧肌肉内。以直接微电极法和组化伞形酮法测定肿瘤组织的 ｐ Ｈ 值，以放射自显影法测定局部放射性强度，经 ３０％ ～４０％ 葡萄糖预处理动物模型改变肿瘤组织的 ｐ Ｈ 值。结果：血清测定表明，荷瘤小鼠的葡萄糖水平显著低于正常对照组。给予葡萄糖刺激动态测定，发现荷瘤小鼠组的上升幅度小、下降快，提示肿瘤处的葡萄糖代谢率增高，而直接微电极法与组化法证实肿瘤组织处的ｐ Ｈ 值低于肌肉处；肿瘤处的 ｐ Ｈ 值在葡萄糖预处理组与未处理组之间的差异达到 ０１５～０４６。将 Ｔｃ（Ⅴ） Ｄ Ｍ Ｓ的摄取以百分数表示，未给予葡萄糖处理的肿瘤处与肌肉组织处每克分别为０２４３％ ±００３０％ 和 ０１５７％ ±００６８％ ，二者比值为 １７７；给予葡萄糖 ５ｇ／ｋｇ 预处理组的肿瘤处的摄取值每克为 ０３６２％ ±０１００％ ，加大葡萄糖用量至６ｇ／ｋｇ 时，该值上升至每克 ０７１０％ ±?
Purpose: Tc(V)-DMS can image a variety of tumors, but the imaging mechanism is inconclusive. The purpose of this study was to observe the effect of Tc(V)-DMS on the uptake of glucose-mediated acidosis tumors, in order to confirm the sensitivity of the tumors to p H values when taking the drug. METHODS: Fifteen mice bearing Ehrlich ascites carcinoma cell line were implanted in the inner thigh muscle. The p H value of tumor tissue was measured by direct microelectrode method and histochemical umbelliferone method. The local radioactive intensity was measured by autoradiography. The p H value of the tumor tissue was changed by a 30% to 40% glucose pretreated animal model. Results: Serum measurements showed that glucose levels in tumor-bearing mice were significantly lower than in normal controls. When glucose stimulation was measured dynamically, it was found that the tumor-bearing mice group had a small increase and a rapid decrease, suggesting an increase in the glucose metabolism rate at the tumor site. Direct microelectrode and histochemistry confirmed that the p H value at the tumor tissue was lower than that at the muscle site. The difference in p H values between the glucose pretreatment group and the untreated group reached 015～046. The uptake of Tc(V)-DMS was expressed as a percentage, and 0.243% ± 0. 030% and 0. 157% ± 0. 068% per g of tumor and muscle tissue where glucose was not administered. The ratio between the two values was 177; the uptake value of the tumor in the pretreatment group given glucose 5g/kg was 0362%±0100% per gram. When glucose was increased to 6g/kg, the value rose to Ke 0710% ±?