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目的:探讨大鼠空间辨别性学习记忆活动大鼠脑穹窿基质细胞衍生因子-1(SDF-1)免疫阳性细胞表达的影响。方法:用水迷宫训练SD大鼠建立空间辨别性学习记忆模型,用免疫组化方法和图像分析技术检测大鼠脑穹窿SDF-1免疫阳性细胞的形态变化。结果:对照组大鼠脑穹窿可见少量圆形或椭圆形SDF-1免疫阳性细胞分布。免疫阳性细胞的胞浆呈淡黄色,颗粒状免疫反应产物附于细胞膜内面。游水组大鼠脑穹窿SDF-1免疫阳性细胞的分布及形态与对照组相比均未见明显差异;模型组训练7 d的大鼠脑穹窿SDF-1免疫阳性细胞的形态和分布与对照组相比未见明显差异。训练14 d和21 d的大鼠神经元细胞在形态上以锥体形或三角形为主。在SDF-1免疫阳性细胞的数量表达上,训练21 d的比训练7d和14 d的明显增多。结论:大鼠脑穹窿内SDF-1免疫阳性细胞可能参与空间辨别性学习记忆活动引致的形态学可塑。
Objective: To investigate the effect of SDF-1 immunopositive cells in spatial learning and memory rats. METHODS: Sprague-Dawley rats were used to train spatial learning and memory model of SD rats. The morphological changes of SDF-1 immunopositive cells in rat forebrain were detected by immunohistochemistry and image analysis. Results: A small number of round or oval SDF-1 immunoreactive cells were found in the control group. The cytoplasm of immunopositive cells showed a light yellow color, and the granular immunoreactive products attached to the inner surface of the cell membrane. There was no significant difference in the distribution and morphology of SDF-1 immunopositive cells in the brain fornix of the swimming group compared with the control group. The shape and distribution of SDF-1 immunopositive cells in the brain fornix No significant difference compared to. The neurons cultured in rats for 14 d and 21 d were predominantly pyramidal or triangular in shape. In the number of SDF-1 immunopositive cells, training 21 d than training 7d and 14 d significantly increased. CONCLUSION: SDF-1 immunoreactive cells in rat forebrain may be involved in morphological plasticity induced by spatial discriminatory learning and memory activities.