姊妹染色单体交换(下称SCE)测定方法,国内外报道甚多,主要是分化染色方法的差异。这些方法尚有不足之处,如加温硷处理法,中期分裂相极易漂浮丢失;多种盐溶液处理步骤繁复;荧光染色猝灭太快,标本不能长久保存等。本文介绍采用紫外线照射及柠檬酸三钠盐液同时处理后,经两次吉氏染色方法,能获得清晰满意标本。它具有试剂来源方便、设备条件简单、分化不受制片的新鲜程度限制,可长期保存读片等优点。方法一、人外周血淋巴细胞培养及5′-溴脱氧尿苷(5′-Brdu)掺入:无菌条件下,于培养瓶 Sister chromatid exchange (hereinafter referred to as SCE) determination methods, many reports at home and abroad, mainly the differentiation of staining methods. There are some shortcomings of these methods, such as heating and alkaline treatment method, the mid-split phase easily floating lost; a variety of salt solution processing steps complicated; fluorescence quenching too fast, specimens can not be preserved for a long time. This article describes the use of UV irradiation and citric acid trisodium salt solution at the same time, after two Kyrgyz staining, can be clearly satisfied samples. It has a convenient source of reagents, equipment, simple conditions, the differentiation is not limited by the freshness of production, the advantages of long-term preservation of reading and so on. Method 1, human peripheral blood lymphocyte culture and 5’-bromodeoxyuridine (5’-Brdu) incorporation: sterile conditions, in the culture flask