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目的探讨姜黄素对结肠炎小鼠脾脏树突状细胞(DC)亚群及其表面活性分子的影响。方法雄性C57小鼠40只,随机分成正常组、200 mg/kg姜黄素组,300 mg/kg美沙拉嗪对照组。三硝基苯磺酸(TNBS)/乙醇法制备结肠炎小鼠模型。给药7 d后,处死小鼠,分离结肠测量其长度并称取其质量,取小鼠脾脏并分离DC,采用流式细胞术检测结肠炎小鼠CD205~+、CD4~+CD205~+、CD8~+CD205~+DC和表面分子主要组织相容性复合体Ⅱ(MHCⅡ)、Toll样受体2(TLR2)、TLR4、CD83的表达情况。同时观察其结肠病理损伤。结果经姜黄素治疗后,与模型组相比,结肠炎小鼠结肠质量指数明显下降,而结肠长度延长,病理性损伤明显缓解,同时其脾脏CD205~+、CD4~+CD205~+、CD8~+CD205~+DC亚群数量下降及其TLR2、TLR4、MHCⅡ的表达明显抑制,而CD83表达水平则显著增高。结论姜黄素治疗小鼠结肠炎可与调节结肠炎小鼠DC亚型比例及其表面共刺激分子的表达有关。
Objective To investigate the effect of curcumin on the subsets of dendritic cells (DCs) and their surface active molecules in colitis mice. Methods Forty male C57 mice were randomly divided into normal group, 200 mg / kg curcumin group and 300 mg / kg mesalazine control group. Colitis mouse model was prepared by trinitrobenzene sulfonic acid (TNBS) / ethanol method. After 7 days of administration, the mice were sacrificed, the length of the colon was isolated, the length of the colon was measured, the quality of the colon was measured, the spleens of mice were taken out and the DCs isolated. The expressions of CD205 ~ +, CD4 ~ + CD205 ~ +, CD8 ~ + CD205 ~ + DC and the expression of major histocompatibility complex (MHC Ⅱ), Toll like receptor 2 (TLR2), TLR4 and CD83 on the surface. At the same time observe the pathological damage of colon. Results After treatment with curcumin, the colon mass index of colitis mice was significantly decreased compared with the model group, while the colon length was prolonged and the pathological injury was relieved. At the same time, the CD205 ~ +, CD4 ~ + CD205 ~ +, CD8 ~ + Decreased the number of CD205 ~ + DC subsets and the expression of TLR2, TLR4 and MHCⅡ, while the expression of CD83 was significantly increased. Conclusion The treatment of colitis with curcumin may be related to the proportion of DC subtypes and the expression of co-stimulatory molecules on the surface of colitis mice.