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背景:前期研究表明透骨消痛胶囊能有效治疗膝骨性关节炎,且对膝关节软骨有保护作用,软骨下骨重塑在骨性关节炎病理进程中起重要作用。目的:观察透骨消痛胶囊干预骨性关节炎软骨下骨重塑的作用及并探讨其作用机制。方法:新西兰大白兔分为正常组、模型组、壮骨关节丸组、透骨消痛胶囊组4组。除正常组外动物均按改良Hulth法造模。正常组、模型组兔每天给予生理盐水灌胃;壮骨关节丸组兔每天给予壮骨关节丸水溶液灌胃;透骨消痛胶囊组兔每天给予透骨消痛胶囊水溶液灌胃。结果与结论:造模后6,9,12周,透骨消痛胶囊组软骨下骨CyclinD1基因、胰岛素样生长因子1、细胞核因子κB受体活化因子配体mRNA表达均显著高于正常组(P<0.05)。与模型组比较,造模后1周,透骨消痛胶囊组Cyclin D1 mRNA表达较低(P<0.05);造模后6周,透骨消痛胶囊组细胞核因子κB受体活化因子配体表达较低(P<0.05);造模后9,12周,透骨消痛胶囊组各项指标表达均较低(P<0.05)。与壮骨关节丸组比较,造模1周后,透骨消痛胶囊组Cyclin D1 mRNA表达较低(P<0.05);6周时透骨消痛胶囊组细胞核因子κB受体活化因子配体表达较低(P<0.05);9,12周后,透骨消痛胶囊组胰岛素样生长因子1mRNA表达较低(P<0.05)。提示透骨消痛胶囊可改变软骨下骨重塑的速率和模式,最终减轻软骨下骨硬化。
BACKGROUND: Previous studies have shown that the traditional Chinese medicine TouJiaoJiaoTong Capsule can effectively treat knee osteoarthritis and protect the knee articular cartilage. Subchondral bone remodeling plays an important role in the pathogenesis of osteoarthritis. Objective: To observe the effect of “Tougu Xiao Tong” capsule on subchondral bone remodeling of osteoarthritis and to explore its mechanism. Methods: New Zealand white rabbits were divided into four groups: normal group, model group, Zhuanggukuanjiewan group and. In addition to normal group of animals were modified Hulth method modeling. Normal group and model group rabbits were given normal saline intragastrically every day. Zhuanggou capsule group was administered with Zhuanggong capsule once a day by intragastric administration. RESULTS AND CONCLUSION: The expression of CyclinD1, IGF-1 and NF-κB ligand mRNA in the subchondral bone of the Weigu Qiao Teng Capsule group were significantly higher than those in the normal group at 6, 9 and 12 weeks P <0.05). Compared with the model group, the expression of Cyclin D1 mRNA in the decoction of Huogu Qiao Tong Capsule was lower than that of the model group (P <0.05) one week after the model was established. At 6 weeks after the model was established, the expressions of Cyclin D1 mRNA, (P <0.05). At 9 and 12 weeks after the model was established, the expression of each index in the Tonggui Qiao Capsule group was lower (P <0.05). Compared with Zhuanggu joint bolus group, the expression of Cyclin D1 mRNA in Huoguxiao Teng capsule group was lower than that in the other two groups (P <0.05) after 1 week of modeling. At 6 weeks, the expression of Cyclin D1 (P <0.05). After 9 and 12 weeks, the expression of insulin-like growth factor 1 mRNA in the decoction group was lower (P <0.05). It is suggested that Huatuo Xiao Tong Capsule can change the rate and mode of subchondral bone remodeling and finally reduce subchondral bone sclerosis.