同源盒基因 (homeobox genes)在植物 ,动物 ,真菌的广泛存在说明这种结构在真核生物进化过程中高度保守 ,并暗示其具有重要功能 .本文将玉米 kn- 1同源盒插入表达载体 p GEMΔ Xba ,并将形成的重组克隆p GEMΔ MK转化 BL 2 1(DE3)而获得高效表达 ;表达产物的分子量为 2 9× 10 3,并主要以可溶性蛋白形式存在 .Kn-1,Quox|1,OSIH- 1间的交叉免疫反应证实三者在蛋白质水平具同源性 .而 Kn- 1同源结构域 (Homeodomain)高效表达有助于进一步寻找其靶基因 The widespread presence of homeobox genes in plants, animals and fungi indicates that this structure is highly conserved during evolution of eukaryotes and implies that it has important functions.In this paper, the maize kn-1 homology box was inserted into the expression vector p GEMΔXba and the resulting recombinant clone p GEMΔMK was transformed into BL 2 1 (DE3) for high expression.The expressed product had a molecular weight of 29 × 10 3 and mainly existed as soluble protein.Kn-1, Quox | 1, OSIH- 1 cross-immunization confirmed that the three have homology at the protein level.High expression of Kn-1 homology domain (Homeodomain) helps to further find its target gene