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目的:探讨大黄素对大鼠肝移植术后急性排斥反应的抑制作用。方法:建立LEW→BN大鼠肝移植动物模型,随机分为A组(排斥反应组)、B组(CsA组)、C组(大黄素组)3组,每组15只大鼠,术后每天分别腹腔注射生理盐水0.5ml/d、环孢素A(CsA)10.0mg·kg-1·d-1、大黄素50.0mg·kg-1·d-1,连续用药8天,停药后每组处死6只大鼠取肝脏组织观察急性排斥活动指数(RAI)和肝细胞凋亡指数(AI),其余大鼠继续饲养以观察存活期。结果:A、B、C组大鼠存活期(天)分别为9.50±1.64、21.57±2.15、21.29±2.21,B、C组存活期较A组明显延长(P<0.01),B、C组间存活期无明显差别(P>0.05)。A、B、C组RAI分别是7.67±0.98、5.17±0.40、5.83±0.75,AI分别是35.83±2.32、15.83±1.33、16.50±2.35。B、C组的RAI、AI较A组均明显降低(P<0.01),B、C组间差别均无意义(P>0.05)。结论:大黄素能有效减少大鼠肝移植术后肝细胞的凋亡,抑制肝移植术后急性排斥反应。
Objective: To investigate the inhibitory effect of emodin on acute rejection after liver transplantation in rats. METHODS: Animal models of LEW→BN rat liver transplantation were established. They were randomly divided into three groups: group A (rejection group), group B (group CsA), and group C (emodin group), 15 rats in each group. Daily intraperitoneal injection of physiological saline 0.5ml/d, cyclosporine A (CsA) 10.0mg·kg-1·d-1, emodin 50.0mg·kg-1·d-1, continuous medication for 8 days, after drug withdrawal Six rats in each group were killed and liver tissues were taken to observe acute rejection activity index (RAI) and hepatocyte apoptosis index (AI). The remaining rats were continuously reared to observe survival. Results: The survival period (days) of rats in groups A, B, and C was 9.50±1.64, 21.57±2.15, and 21.29±2.21, respectively. The survival time of group B and C was significantly longer than that of group A (P<0.01). There was no significant difference in inter-term survival (P>0.05). The RAI of groups A, B, and C were 7.67±0.98, 5.17±0.40, and 5.83±0.75, respectively, and the AI was 35.83±2.32, 15.83±1.33, and 16.50±2.35, respectively. The RAI and AI in group B and C were significantly lower than those in group A (P<0.01). There was no significant difference between group B and group C (P>0.05). Conclusion: Emodin can effectively reduce the apoptosis of liver cells after liver transplantation in rats and inhibit the acute rejection after liver transplantation.