本文研究了金针菇菌丝体中多糖的提取、纯化和分子量分布及其测定。样品用pH8～9的弱碱性水溶液于室温下提取水溶性多糖,经三次提取已基本完全。提取液经浓缩、醇析、离心、干燥后得粗多糖,得率为7.07％。粗多糖再经Savage法脱蛋白、再次醇析后得灰白色纯多糖,得率为0.6％。纯多糖用高效液相色谱法分离得到四个色谱峰,它们的分子量分别为1,930,000、574,000、56,000和15,000。 In this paper, the extraction, purification, molecular weight distribution and determination of polysaccharides from Flammulina velutipes mycelia were studied. The sample was extracted with a weakly basic aqueous solution of pH8 ~ 9 at room temperature, and the water-soluble polysaccharide was extracted three times and was almost completely extracted. The extract was concentrated, alcoholysis, centrifugation, drying crude polysaccharides, the yield was 7.07%. Crude polysaccharides and protein by Savage deproteinization, again after alcoholysis was pure white polysaccharides, yield was 0.6%. Pure polysaccharides were separated by high performance liquid chromatography four peaks, their molecular weights were 1,930,000,574,000,56,000 and 15,000.