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应用透射电子显微镜观察了生长在含有2,4-D2mg/L的N_6。诱导培养基上和生长在不含2,4-D而含有NAA0.5mg/L和KT0.5mg/L的MS分化培养基上的水稻花药培养的愈伤组织。生长在诱导培养基上的愈伤组织是异质性的。当愈伤组织由诱导培养基上转移到分化培养基上2天后,细胞超微结构有明显变化。随着器官分化的进程,有些愈伤组织的细胞要经历一个质体、线粒体、内质网及核糖体大量增多的代谢活跃期,为新的形态建成提供物质和能量,本试验还观察到质体、线粒体以缢缩的方式分裂增殖。
Transmission electron microscopy was used to observe the growth of 2,4-D2mg / L containing N_6. Callus cultured on rice induction medium and on MS differentiation medium containing 2,4-D without NAA 0.5 mg / L and KT 0.5 mg / L. Callus growing on induction medium is heterogeneous. When the callus was transferred from the induction medium to differentiation medium for 2 days, the ultrastructure of the cells changed significantly. With the process of organ differentiation, some callus cells undergo a massively active metabolic phase of plastids, mitochondria, endoplasmic reticulum and ribosomes, providing material and energy for new morphogenesis. In this experiment, Body, mitochondria to shrink way to proliferate.