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取冻干的新鲜白花曼陀罗(Datura metel)和毛叶曼陀罗(D.innoxia)的叶,分 别以pH7.5,0.02M Tris-盐酸缓冲液1.5L制成匀浆,匀浆经2层布筛过滤后离心,上清液经阴离子交换树脂(DEAE-纤维素)和
The freeze-dried fresh leaves of Datura metel and D. innoxia were homogenized with pH 7.5 and 0.02 M Tris-HCl buffer 1.5 L respectively. After 2 layers of sieves were filtered and centrifuged, the supernatant was passed through an anion exchange resin (DEAE-cellulose) and