Growth and uptake of exogenous phosphate by Microcystis aeruginosa in batch culture under differenttemperature, photoperiod, and turbulence were studied by the method of phosphate isotope tracer. Relatively hightemperature, long photoperiod and strong turbulence increased the cell density of M. aeruginosa in these batchcultures. The initial rapid uptake of phosphate by M. aeruginosa was independent of the temperature, photoperiod,and turbulence. Similarly, maximum exogenous phosphate uptake was not related to these environmental factors.However, elevated temperature and turbulence shortened the time, required to obtain maximum P accumulation.The growth of M. aeruginosa could alleviate the phosphorous leakage. Total amounts of exogenous phosphateuptake to M. aeruginosa and the phosphorus leakage of M. aeruginosa were significantly influenced by the growthstate of M. aeruginosa closely correlated with the environmental factors. The maximum volume of exogenousphosphate uptake to M. aeruginosa was 46% of added exogenous phosphate in water with 16 hours of photoperiod.Thus, total amounts of exogenous phosphate uptake to M. aeruginosa were more strongly affected by thephotoperiod length than temperature and turbulence.