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目的:探讨HIF-1α、AQP-1表达对宫颈鳞状细胞癌血管生成的作用。方法:应用免疫组织化学方法检测54例宫颈鳞状细胞癌中HIF-1α、AQP-1的表达,用CD105标记肿瘤微血管密度。结果:HIF-1α在宫颈鳞状细胞癌的阳性表达率为50%,表达水平与分化程度、浸润深度明显相关(r=-0.674,P<0.05和r=0.897,P<0.01),与临床分期、肿瘤体积、淋巴结转移无明显相关(P>0.05)。AQP-1在宫颈鳞状细胞癌的表达率为87.1%,表达水平与肿瘤体积、临床分期明显相关(r=0.989,P<0.05和r=0.807,P<0.01),与分化程度、淋巴结转移、浸润深度无明显相关(P>0.05)。微血管密度与肿瘤体积、浸润深度明显相关(r=0.609,P<0.05和r=0.845,P<0.01);与临床分期、分化程度、淋巴结转移无明显相关(P>0.05)。微血管密度与HIF-1α表达明显相关(r=0.701,P<0.05),而与AQP-1的表达无关(P>0.05)。AQP-1的表达水平与HIF-1α的表达水平明显相关(r=0.648,P<0.05)。结论:HIF-1α、AQP-1均参与宫颈鳞状细胞癌缺氧诱导新生血管过程,并且与微血管密度密切相关,其中HIF-1α发挥重要作用。
Objective: To investigate the effect of HIF-1α and AQP-1 on angiogenesis in cervical squamous cell carcinoma. Methods: The expressions of HIF-1α and AQP-1 in 54 cases of cervical squamous cell carcinoma were detected by immunohistochemistry. The density of tumor microvessels was marked by CD105. Results: The positive rate of HIF-1α in cervical squamous cell carcinoma was 50%. The expression level of HIF-1α was significantly correlated with the degree of differentiation and depth of invasion (r = -0.674, P <0.05 and r = 0.897, P <0.01) Staging, tumor volume and lymph node metastasis had no significant correlation (P> 0.05). The expression of AQP-1 in cervical squamous cell carcinoma was 87.1%. The expression level of AQP-1 was significantly correlated with tumor volume and clinical stage (r = 0.989, P <0.05 and r = 0.807, P <0.01) , Infiltration depth no significant correlation (P> 0.05). Microvessel density was significantly correlated with tumor volume and depth of invasion (r = 0.609, P <0.05 and r = 0.845, P <0.01), but not with clinical stage, differentiation and lymph node metastasis (P> 0.05). Microvessel density was positively correlated with HIF-1α expression (r = 0.701, P <0.05), but not with AQP-1 expression (P> 0.05). The expression of AQP-1 was significantly correlated with the expression of HIF-1α (r = 0.648, P <0.05). Conclusion: Both HIF-1α and AQP-1 are involved in hypoxia-induced neovascularization in cervical squamous cell carcinoma and are closely related to the microvessel density, of which HIF-1α plays an important role.