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[目的]探讨BRMS1mRNA在乳腺癌中的表达及其与乳腺癌临床病理特征的关系。[方法]采用Trizol提取组织总RNA,将mRNA转录成cDNA,用实时荧光定量聚合酶链反应(RFQ-PCR)技术检测乳腺组织BRMS1mRNA表达,以BRMS1mRNA和GAPDHmRNA含量的比值表示BRMS1表达水平。[结果]乳腺癌组织中BRMS1mRNA的表达水平明显低于癌旁组织(P<0.01),也明显低于正常乳腺组织(P<0.01),BRMR1mRNA低表达与临床病理分期和淋巴结转移有关(P<0.05,P<0.01),而与患者年龄、癌肿大小、病理类型无关(P>0.05)。[结论]乳腺癌组织中BRMS1mRNA的低表达与乳腺癌的发展与转移有关,可能成为判断乳腺癌转移的一个标志物。
[Objective] To investigate the expression of BRMS1 mRNA in breast cancer and its relationship with the clinicopathological features of breast cancer. [Method] Trizol was used to extract tissue total RNA and transcribed into cDNA. The expression of BRMS1 mRNA was detected by real-time fluorescent quantitative polymerase chain reaction (RFQ-PCR), and the ratio of BRMS1 mRNA and GAPDH mRNA was used to express BRMS1. [Results] The expression of BRMS1 mRNA in breast cancer tissues was significantly lower than that in paracancer tissues (P <0.01) and also significantly lower than that in normal breast tissues (P <0.01). The low expression of BRMR1 mRNA was associated with clinical pathological stage and lymph node metastasis (P < 0.05, P <0.01), but not with age, size of cancer and pathological type (P> 0.05). [Conclusion] The low expression of BRMS1 mRNA in breast cancer is related to the development and metastasis of breast cancer, which may be a marker of breast cancer metastasis.