通过研究酶膜反应器系统对酶活的影响,可知酶膜反应器系统对碱性蛋白酶(Alcalase)具有很好的保留效果。蠕动泵运转过程对酶造成的破坏以及超滤膜对酶的吸附是导致碱性蛋白酶酶活损失的主要原因。通过利用Design Expert 6.0.5设计四因素三水平的响应面分析试验,求得酶膜反应器制备燕麦蛋白质ACE抑制肽的最佳工艺条件为底物质量分数3%、加酶量3.5%、料液流量45L/h、操作压力0.07MPa、温度61℃、pH8,在该条件下产物的ACE抑制率可达67.86%。 By studying the influence of enzyme membrane reactor system on enzyme activity, enzyme membrane reactor system has good retention effect on Alcalase. Peristaltic pump damage caused by the operation of the enzyme and ultrafiltration membrane enzyme adsorption is the main cause of alkaline protease enzyme activity loss. Through the design of four-factor and three-level response surface analysis with Design Expert 6.0.5, the best technological conditions for preparing ACE inhibitory peptide from oat protein membrane reactor were obtained as 3% substrate mass fraction and 3.5% enzyme dosage, Liquid flow rate 45L / h, operating pressure 0.07MPa, temperature 61 ℃, pH8, under the conditions of product ACE inhibition rate up to 67.86%.