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本文用免疫组化ＳＡＢＣ技术，动态观察感染日本血吸虫小鼠不同时期肝内ＴＮＦ－α的含量及分布。结果显示感染后第８～１２ｗｋ，肝内ＴＮＦ明显增加，１６ｗｋ达高峰，显著高于第８ｗｋ的水平（Ｐ＜０．００１），主要分布在虫卵肉芽肿内及其周围。在慢性感染期，肝内ＴＮＦ并未随着肝内胶原的增加而增加，反现减少。ＦＮ、ＬＮ、Ⅰ及Ⅲ型胶原于感染后第８ｗｋ开始增加，呈细线状分布在汇管区及肉芽肿周围；并分别于２０及２４ｗｋ达高峰，且增宽变厚，呈条索状或网状沉积在肉芽肿周围及其内。给受染动物注射重组ＴＮＦ后，Ⅰ及Ⅲ型胶原含量较同期非注射组增加，其中Ⅰ型胶原增加较显著（Ｐ＜０．０１）；而给正常小鼠注射ＴＮＦ无类似变化。提示ＴＮＦ能刺激成纤维细胞增生，致肝内胶原分泌增加，但可能需要某些因素的协同作用。
In this paper, immunohistochemical SABC technique was used to dynamically observe the content and distribution of intrahepatic TNF-α in mice infected with Schistosoma japonicum. The results showed that TNF increased significantly at 8th to 12th week after infection and peaked at 16th week, which was significantly higher than that at 8th week (P <0.001). It mainly distributed in and around the ova granuloma. In the chronic phase of infection, intrahepatic TNF did not increase with the increase of intrahepatic collagen, but decreased again. FN, LN, type Ⅰ and type Ⅲ collagen began to increase at the 8th week after infection, showing a fine line distribution around the portal area and granuloma; reached the peak at 20 and 24 weeks, and became wider and thicker, Reticular deposition in and around granulomas. After injection of recombinant TNF into infected animals, the content of collagen type I and type III increased as compared with the non-injection group at the same period, in which the type I collagen increased more significantly (P <0.01), while the normal mice injected with TNF had no similar changes. Tip TNF can stimulate fibroblast proliferation, resulting in increased hepatic collagen secretion, but may need some factors synergistic effect.