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目的：观察RP多糖在体内对DHBV的抑制作用。方法体内实验以先天感染DHBV的雏鸭为动物模型，将雏鸭(10只/组)随机分为5组(RP多糖高、中、低3个剂量组、3-T C组、生理盐水对照组)，均以20m g/k g/d灌胃给药10天。给药前1天、给药后5天、10天及停药后3天，均采用斑点杂交法观察血清中DHBV-DNA表达。结果各剂量组RP多糖对DHBV均有相应的抑制作用。其中给药后第5天，高、中剂量组血清DHBV-DNA为0.77±0.27、0.69±0.15，第10天为0.57±0.11、0.49±0.19，与生理盐水对照组(1.32±0.56、1.24±0.53)相比明显降低。给药后第5天，RP多糖高、中剂量组对DHBV-DNA的抑制率为25.16%、25.35%，第10天为45.94%、48.28%，与生理盐水对照组对比显著提高。结论 RP多糖可降低血DHBV-DNA含量，对DHBV有抑制作用。“,”Objective: To observe the in-vivo inhibitory effect of RP polysaccharide on DHBV.Methods: 1-day old Beijing ducklings with congenital infection of DHBV were used as the animal model and were randomly divided into five groups(Namely, RP polysaccharide high dose group,medium dose group,low dose group,Lamivudine group and normal saline group). Each group of ducks were given the drug with the doses 20mg/kg/d for 10 days. The expression of DHBV-DNA was detected by dot-blot hybridization test 1day before treatment, five days and ten days during treatment, and 3 days after drug withdrawal, respectively. Results: In each group of the RP polysaccharide has corresponding inhibition on DHBV. After being medicated with RP polysaccharide for five days, in RP polysaccharide high dose group, medium dose group, Serum DHBV-DNA was 0.77±0.27,0.69±0.15; while for ten days, 0.57±0.11,0.49±0.19,respectively, which was obviously lower compared with those in normal saline group(1.28±0.54,1.20±0.5l). The inhibition rates of DHBV-DNA in RP polysaccharide high dose group, medium dose group were 25.16%,25.35% respectively 5 days after treatment, while 45.94%,48.28%;respectively 10 days after treatment, indicating remarkable improvement compared with the control group. Conclusion: RP polysaccharide can protect duck liver against inflammation by the inhibition of DHBV.