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目的探讨Yes相关蛋白(YAP)、细胞周期蛋白D1(Cyclin D1)在宫颈癌前病变与宫颈鳞癌中的表达。方法收集100例患者宫颈病变组织,其中宫颈鳞癌35例、CIN48例、正常宫颈组织17例,以免疫组织化学法检测以上组织标本中YAP、Cyclin D1的表达。结果宫颈鳞癌组织、宫颈上皮内瘤变(CIN)组织和正常宫颈组织中YAP阳性表达率分别为65.71%、41.67%、11.76%,Cyclin D1阳性表达率分别为60.00%、33.33%、0.00%,阳性表达率在不同组织中比较差异有统计学意义(P<0.05),YAP、Cyclin D1阳性表达率宫颈鳞癌组织均高于CIN组织及正常宫颈组织(P<0.05),CIN组织高于正常宫颈组织(P<0.05);宫颈鳞癌临床分期III-IVYAP、Cyclin D1阳性表达率为80.00%、75.00%高于Ⅰ~Ⅱ期者46.67%、40.00%(P<0.05),有淋巴结转移者YAP、Cyclin D1阳性表达率为78.26%、73.91%高于Ⅰ~Ⅱ期者41.67%、33.33%(P<0.05);CINⅢ期者YAP、Cyclin D1阳性表达率为55.56%、48.15%高于Ⅱ期者23.81%、14.29%(P<0.05)。结论 YAP、Cyclin D1在宫颈癌前病变与宫颈鳞癌中的表达均有上调,并与病情发生及发展密切相关。
Objective To investigate the expression of Yes-associated protein (YAP) and Cyclin D1 in cervical precancerous lesions and cervical squamous cell carcinoma. Methods 100 patients with cervical lesions were collected, including 35 cases of cervical squamous cell carcinoma, 48 cases of CIN and 17 cases of normal cervical tissue. The expressions of YAP and Cyclin D1 were detected by immunohistochemistry. Results The positive rates of YAP expression in cervical squamous cell carcinoma, cervical intraepithelial neoplasia (CIN) and normal cervical tissues were 65.71%, 41.67% and 11.76%, respectively. The positive rates of Cyclin D1 were 60.00%, 33.33% and 0.003% (P <0.05). The positive expression rates of YAP and Cyclin D1 in cervical squamous cell carcinoma tissues were significantly higher than those in CIN and normal cervical tissues (P <0.05), and the positive expression rate of CIN was higher than that in normal cervical tissues (P <0.05). The positive rates of clinical stage III-IVYAP and Cyclin D1 in cervical squamous cell carcinoma were 80.00%, 75.00%, 46.67% and 40.00% respectively (P <0.05), with lymph node metastasis The positive rates of YAP and Cyclin D1 were 78.26% and 73.91%, respectively, higher than that of stage Ⅰ ~ Ⅱ 41.67% and 33.33% (P <0.05). The positive rates of YAP and Cyclin D1 in CIN stage were 55.56% and 48.15% 23.81% and 14.29% in stage II (P <0.05). Conclusion The expression of YAP and Cyclin D1 in cervical precancerous lesions and cervical squamous cell carcinoma both have an up-regulation, which is closely related to the occurrence and development of the disease.