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为了揭示不同倍性小麦适应盐胁迫的差异,本实验以人工合成六倍体(AABBDD)小麦及其四倍体(AABB)小麦(Triticum turgidum)和二倍体(DD)节节麦(Aegilops tauschii)亲本为实验材料,研究了不同浓度Na Cl(0、200 mmol·L-1)胁迫处理下小麦幼苗K+、Na+含量以及K+/Na+的变化规律,以及不同浓度(0、50、100、200 mmol·L-1)盐胁迫对超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、丙二醛(MDA)含量、脯氨酸含量、可溶性蛋白和可溶性糖含量的影响规律。结果表明,四倍体表现出显著的高Na+低K+以及较低的K+/Na+,二倍体表现出显著的低Na+高K+和较高的K+/Na+,Na Cl胁迫时离子含量变化大,对盐胁迫的适应性更强,六倍体在积累K+的能力上也有一定的优势。低浓度(50-100mmol·L-1)盐胁迫使3种倍性材料的丙二醛含量和抗氧化酶活性升高。四倍体在累积渗透调节物质和调节抗氧化酶的活性的能力上显著强于二倍体和六倍体,六倍体在POD活性以及积累脯氨酸和可溶性蛋白的能力上也具有一定的优势。根据研究结果推测,含有DD染色体组的二倍体节节麦主要通过调节K+/Na+来适应盐胁迫,而含有AABB染色体组的四倍体小麦主要通过调节抗氧化酶的活性累积渗透调节物质来适应盐胁迫,作为二倍体和四倍体远缘杂种的人工合成六倍体小麦则表现出了综合的耐盐适应性机制,相较于两亲本具有更加广泛耐盐适应性。
In order to reveal the difference of salt stress between different ploidy varieties, we constructed a new type of wheat cultivars AABBDD, AABB Triticum turgidum and Aegilops tauschii ) Parents were used as experimental materials to study the changes of K +, Na + and K + / Na + contents of wheat seedlings under different concentrations of NaCl (0, 200 mmol·L-1) The effects of salt stress on the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), malondialdehyde (MDA), proline content, soluble protein The influence of soluble sugar content. The results showed that the tetraploid showed significant high Na + low K + and low K + / Na +, diploid showed significant low Na + K + and high K + / Na +, NaCl stress ion content changes, Salt stress adaptability stronger, hexaploid in the accumulation of K + also have some advantages. Under the salt stress of low concentration (50-100mmol·L-1), the content of malondialdehyde and antioxidant enzyme activity of three ploidy materials increased. Tetraploids were significantly more potent than diploid and hexaploid in their ability to accumulate osmolytes and regulate antioxidant enzymes, and hexaploid also had some POD activity and ability to accumulate proline and soluble proteins Advantage. According to the results of the study, it was speculated that diploid nodules containing DD genome could adapt to salt stress through regulating K + / Na +, while tetraploid wheat containing AABB genome mainly through the regulation of the activity of osmolytes Adapted to salt stress, hexaploid wheat as diploid and tetraploid distant hybrids showed a comprehensive mechanism of salt-tolerance adaptation, which had more extensive salt tolerance than the parents.