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目的:研究田基黄苷(Quercetin-7-β-D-rhamnose)抗大鼠肝纤维化作用。方法:采用胆总管结扎法制备肝纤维化大鼠模型;从造模当天起,腹腔注射田基黄苷(0.5、1.0、2.0 mg/kg),给药4 w后,测定血清总胆红素(TBIL)、直接胆红素(DBIL)、丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、TNF-α水平及肝组织中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)和羟脯氨酸(Hyp)的含量;采用HE染色观察肝细胞结构和肝纤维化程度;采用免疫组织化学法检测肝组织α-肌动蛋白(α-SMA)的表达。结果:田基黄苷能显著降低胆总管结扎诱导的肝纤维化大鼠血清TBIL、DBIL、ALT、AST、PC-Ⅲ、HA、LN的水平及肝组织中Hyp的含量。此外,也能提高肝组织SOD、GSH-Px的活性,降低肝组织MDA及血清中TNF-α的含量。病理组织观察结果显示:田基黄苷组大鼠肝小叶结构较清晰,肝内小胆管以汇管区为中心轻度增生,增生胆管周围伴轻度纤维组织增生,肝组织胶原纤维沉积显著减轻;且肝组织α-SMA蛋白表达量减少。结论:田基黄苷能抑制胆总管结扎诱导的大鼠肝纤维化的形成,其抗肝纤维化作用与其抗氧化作用及抗TNF-α的分泌有关。
Objective: To study the anti-liver fibrosis effect of Quercetin-7-β-D-rhamnose in rats. Methods: The model of hepatic fibrosis was established by common bile duct ligation. From the day of modeling, intraperitoneal injection of rhubarb glycosides (0.5,1.0,2.0 mg / kg), 4 w after administration, serum total bilirubin (TBIL), direct bilirubin (DBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN) (PCⅢ), TNF-α and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and hydroxyproline (Hyp) The hepatic cell structure and hepatic fibrosis were observed by HE staining. The expression of α-SMA in liver tissue was detected by immunohistochemistry. Results: Tianhuang Huang glycosides can significantly reduce the level of serum TBIL, DBIL, ALT, AST, PC-Ⅲ, HA and LN and the level of Hyp in liver tissues induced by common bile duct ligation in rats with liver fibrosis. In addition, it can also improve the activity of SOD and GSH-Px in liver tissue and decrease the contents of MDA and serum TNF-α in liver tissue. Histopathological examination showed that the structure of hepatic lobules in trazmalin group was clearer, mild intrahepatic biliary duct hyperplasia centered in portal area, hyperplasia of bile ducts with mild fibrosis and collagen deposition in liver tissue were significantly reduced. And hepatic α-SMA protein expression decreased. Conclusion: TXT can inhibit the formation of hepatic fibrosis in rats induced by common bile duct ligation, and its anti-hepatic fibrosis is related to its anti-oxidation and anti-TNF-α secretion.