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采用UV - 91 0 0型紫外可见分光光度计 ,测定了由植物油脂制得的共轭亚油酸、共轭亚油酸甲酯、共轭亚油酸三甘酯在不同浓度时在 2 0 0nm - 30 0nm的吸光度 ,绘制出样品在不同浓度时的吸光度曲线 ,找出适宜的浓度范围 ,用MicrocalSoftwareOriginVersion 4.0做回归分析 ,由线性回归给出样品浓度和吸光度的线性关系 ,为对实验结果进行检验和校正 ,采用惠普 845 2A二极管阵列分光光度计测定了同一批样品在不同浓度范围内的吸光度 ,利用PEAutosystemXL -TurboMass测得样品中共轭亚油酸准确浓度 ,结合回归系数给出了用紫外可见分光光度计快速测定样品中共轭亚油酸含量的经验公式
The conjugated linoleic acid, conjugated linoleic acid methyl ester and conjugated linoleic acid triglyceride were determined by UV - 91 0 0 UV - Vis spectrophotometer at different concentrations of 20 0nm - 300nm absorbance, draw the absorbance curve of the sample at different concentrations to find the appropriate concentration range, with MicrocalSoftwareOriginVersion 4.0 regression analysis, the linear regression gives the linear relationship between sample concentration and absorbance, the experimental results for the Test and calibration, the HP 845 2A diode array spectrophotometer was used to determine the absorbance of the same batch of samples in different concentration range. The exact concentration of conjugated linoleic acid in the sample was measured by PE Autosystem XL-Turbo Mass Spectrometry, and the correlation coefficient The Empirical Formula of Rapid Determination of Conjugated Linoleic Acid in Sample by Spectrophotometer