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利用RSAP标记技术对18份皂荚种质材料进行了遗传多样性分析。结果表明,从45对RSAP引物中筛选了12对引物进行PCR扩增,共扩增出167个条带,其中多态性条带154个,多态性位点百分率(PPL)为92.22%。各引物Nei’s基因多样性指数(H)和Shannon’s信息指数(I)的平均值分别为0.229 8和0.371 6,18份皂荚种质的遗传相似系数(GS)为0.431 1~0.988 0。UPGMA聚类分析表明,在GS为0.62处可将18份皂荚种质资源分为3组,其中,野皂荚单独为一组,山皂荚和皂荚-T聚为一组,其他皂荚材料聚为一组。利用6对引物扩增的9个多态性位点,构建了18份皂荚种质资源的DNA指纹图谱,可以将其区分并精准鉴定。
The genetic diversity of 18 Acacia germplasms was analyzed by RSAP marker. Twelve pairs of primers were screened from 45 pairs of RSAP primers for PCR amplification. A total of 167 bands were amplified, of which 154 were polymorphic. The percentage of polymorphic loci (PPL) was 92.22%. The genetic diversity index (H) and Shannon’s information index (I) of each primer Nei’s were 0.229 8 and 0.371, respectively. The genetic similarity coefficient (GS) of 18 and 18 accessions ranged from 0.431 1 to 0.988 0. UPGMA cluster analysis showed that 18 GSHs could be divided into three groups at GS 0.62, among which wild camellia pods were grouped as a group, group. Nine polymorphic sites amplified by six pairs of primers were used to construct 18 DNA fingerprints of Acacia germplasm resources, which can be distinguished and accurately identified.