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应用黄嘌呤氧化酶（Ｈｙｐｏｘａｎｔｈｉｎｅ－ＸａｎｔｈｉｎｅＯｘｉｄａｓｅＨＸＯ）氧自由基体系和微管吸吮方法（ＭｉｃｒｏｐｉｐｅｔｔｅＡｓｐｉｒａｔｉｏｎＭｅｔｈｏｄ），研究氧自由基对红细胞膜蛋白巯基氧化损伤，引起膜蛋白组成的变化，导致膜粘弹性参数膜弹性模量μ和粘性系数η发生变化。实验结果表明：（１）随着氧化酶浓度的增加，膜蛋白巯基的含量下降，膜蛋白交联形成高分子量组分（ＨＭＢ）；（２）膜弹性模量μ和粘性系数η随膜蛋白巯基含量的下降而上升；（３）膜蛋白巯基含量与膜弹性模量μ和粘性系数η间有显著的定量相关性。分析探讨了引起红细胞膜力学特性变化的膜蛋白巯基基团反应机制。
The hypoxanthine oxidase (Hypoxanthine-Xanthine OxidaseHXO) oxygen free radical system and the microtubule aspiration method were used to study the oxidative damage of erythrocyte membrane protein by thiol-oxygen and the change of membrane protein composition. The amount μ and the viscosity coefficient η change. The experimental results showed that: (1) With the increase of the concentration of oxidase, the content of sulfhydryl in membrane protein decreased and the membrane protein crosslinked to form high molecular weight component (HMB); (2) The membrane modulus of elasticity (3) There was a significant quantitative correlation between the membrane thiol content and the membrane elastic modulus μ and the viscosity coefficient η. The membrane protein thiol group reaction mechanism that caused the change of mechanical properties of erythrocyte membrane was analyzed.