论文部分内容阅读
目的探讨妊娠期应用低分子质量肝素(LMWH)对新生鼠肝细胞先天性感染人巨细胞病毒(HCMV)的病理改变和病毒;;DOI:10.3969/j.issn.NA载量的影响。方法取健康纯系清洁级Balb/c小鼠48只。随机选择配对,每对雌雄小鼠各1只。实验组20对,正常对照组4对。实验组每4对为1组,共5组,每只分别腹腔内注射6.0 log半数组织培养感染剂量(TCI;;DOI:10.3969/j.issn.50)HCMV-A;;DOI:10.3969/j.issn.169。正常对照组4对,每只腹腔内注射相应细胞维持液高糖改良Eagles培养液(;;DOI:10.3969/j.issn.MEM)1.0 mL。所有小鼠于接种后配对,隔离喂养,并观察受精情况,记录受精时间。发现妊娠后,分别予实验Ⅰ组皮下注射LMWH1 000 U.kg-1,实验Ⅱ组皮下注射LMWH500 U.kg-1,实验Ⅲ组皮下注射LMWH250 U.kg-1,实验Ⅳ组肌肉注射更昔洛韦(GCV)60 mg.kg-1,各10 d;实验Ⅴ组为阳性对照组,孕鼠不注射LMWH和GCV。每组随机取10只新生鼠,出生1 d处死,取出肝脏,进行病理学检查和荧光定量PCR检测。结果实验Ⅰ组肝组织病理损害较阳性对照组明显减轻,HCMV;;DOI:10.3969/j.issn.NA载量[(1.60±1.12)×103拷贝数/50 mg]较阳性对照组[(5.91±2.91)×107拷贝数/50 mg]显著降低(P<0.01),与实验Ⅳ组[(1.75±1.05)×103拷贝数/50 mg]比较差异无统计学意义(P>0.05)。实验Ⅱ组肝组织炎性反应较阳性对照组稍减轻。实验Ⅲ组肝组织炎性反应与阳性对照组比较无明显变化,实验Ⅲ组HCMV ;;DOI:10.3969/j.issn.NA载量为(5.49±2.76)×107拷贝数/50 mg,与阳性对照组比较差异无统计学意义(P>0.05)。结论在妊娠期给予LMWH1 000 U.kg-1干预能显著降低HCMVA;;DOI:10.3969/j.issn.169株先天性感染Balb/c新生鼠肝细胞病毒感染和复制,减轻细胞炎性反应。
Objective To investigate the pathological changes and virus infection of human cytomegalovirus (HCMV) in neonatal rat hepatocytes infected with low molecular weight heparin (LMWH) during pregnancy. Methods Pure healthy clean grade Balb / c mice 48. Paired randomly, each male and female mice each one. 20 in the experimental group and 4 in the normal control group. The experimental group was divided into 4 groups with 1 in each group, with a total of 5 groups. Each group was intraperitoneally injected with 6.0 log half tissue culture inoculation dose (TCI ;; DOI: 10.3969 / j.issn.50); HCMV-A ;; DOI: 10.3969 / j .issn.169. Normal control group of 4 pairs, each intraperitoneal injection of the corresponding cell maintenance solution high glucose Eagles culture medium ;; DOI: 10.3969 / j.issn.MEM) 1.0 mL. After inoculation, all mice were paired, fed separately, and observed fertilization, recording fertilization time. Found after pregnancy, respectively, to the experimental group Ⅰ subcutaneous injection of LMWH1 000 U.kg-1, experimental group Ⅱ subcutaneous LMWH500 U.kg-1, experimental group Ⅲ subcutaneous LMWH250 U.kg-1, experimental group IV intramuscular injection of the past (GCV 60 mg.kg-1, each 10 d); experimental group V was positive control group, pregnant rats were not injected with LMWH and GCV. Ten newborn rats were randomly selected in each group and sacrificed at 1 day after birth. The liver was removed for pathological examination and real-time quantitative PCR. Results Compared with the positive control group, the histopathological damage of experimental group Ⅰ was significantly reduced compared with that of the positive control group (HCMV ;; DOI: 10.3969 / j.issn.NA load [(1.60 ± 1.12) × 103 copies / 50 mg] ± 2.91) × 107 copies / 50 mg] (P <0.01). There was no significant difference between the experimental group and the control group [(1.75 ± 1.05) × 103 copies / 50 mg] (P> 0.05). Experimental group Ⅱ liver inflammation than the positive control group slightly reduced. There was no significant difference in inflammatory reaction between the experimental group and the positive control group. The load of HCMV and DOI: 10.3969 / j.issn.NA in experimental group Ⅲ was (5.49 ± 2.76) × 107 copies / 50 mg, There was no significant difference in the control group (P> 0.05). Conclusions Intervention with LMWH1000 U.kg-1 during pregnancy can significantly reduce HCMVA ;; DOI: 10.3969 / j.issn.169 Infection and replication of Balb / c neonatal murine hepatoma virus congenital infection, reduce cellular inflammatory response.