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分别取2、4、6、8年前种植过地黄的土壤进行大田再植生物测试,并采用HPLC检测再植地黄土壤中与化感现象密切相关的5种酚酸(阿魏酸、对羟基苯甲酸、香草酸、香豆酸和丁香酸)的含量。结果表明:随着土壤间隔年限的增加,大田再植地黄的块根干质量、体积和叶片干质量依次增加,且从地黄苗期就开始表现出这种变化趋势;地黄土壤中5种酚酸的含量也依次降低,间隔2、4、6、8年的再植地黄土壤中5种一元酚酸总含量分别为1.503、1.323、0.700和0.340μg.g-1。间隔2、4、6、8年的再植地黄土壤水提液中阿魏酸、对羟基苯甲酸和香豆酸含量与地黄大田生物测试结果呈显著或极显著负相关。从整体看,地黄生育的前期和中期应当是地黄化感作用的重点时期。
The soil of Rehmannia glutinosa planted in two, four, six and eight years ago was bio-tested for replanting, and five kinds of phenolic acids (ferulic acid, hydroxyl group Benzoic acid, vanillic acid, coumaric acid and syringic acid). The results showed that the root mass, dry weight and dry weight of Rehmannia glutinosa rhizome were increased with the increase of soil interval, and showed the tendency of change from the seedling stage of Rehmannia glutinosa. The contents of five phenolic acids The content of five kinds of monophenolic acids in the reed rhizomes at intervals of 2, 4, 6 and 8 years were 1.503, 1.323, 0.700 and 0.340μg.g-1, respectively. The contents of ferulic acid, p-hydroxybenzoic acid and coumaric acid in the retilation water solution of Rehmannia glutinosa relict at intervals of 2, 4, 6 and 8 years were significantly or very significantly negatively correlated with the bioassay results of Rehmannia glutinosa. As a whole, the early and mid-term reproductive rehmannia should be the key period of allelopathic effect.