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目的:分析慢性胃炎胃液细菌学分布,探讨其临床意义。方法:对慢性胃炎患者,胃镜下无菌操作采取胃液,涂片染色显微镜检查;对有感染的患者胃液进行细菌培养、分离,利用珠海黑马生物Bact-IST进行鉴定及药敏检测;用Whonet 5.0软件进行数据分析。结果:胃液镜检与细菌培养阳性率随慢性胃炎患病时间延长而增加;从胃液标本中分离出细菌阳性109例,阳性率为79.56%(109/137);所分离到的革兰阴性杆菌占78.90%(86/109),革兰阳性球菌占39.45%(43/109),真菌占19.27%(21/109);其中混合感染占27.52%(30/109)。在革兰阴性杆菌中,发酵菌占72.10%(62/86),主要有大肠埃希菌、肺炎克雷伯菌、肠杆菌、枸橼酸杆菌;非发酵菌占27.90%(24/86),包括绿脓假单胞菌、不动杆菌、嗜麦芽窄食假单胞菌。结论:慢性胃炎患者早期进行细菌学鉴定有助于临床治疗。
Objective: To analyze the bacteriological distribution of gastric juice in chronic gastritis and to discuss its clinical significance. Methods: Gastric fluid was obtained under atrophic gastroscopy and stained with smear microscopy. The gastric juice of infected patients was cultured and separated. The samples were identified by using Bact-IST from Zhuhai Herb. Software for data analysis. Results: The positive rates of gastroscopy and bacterial culture increased with the prolongation of chronic gastritis. The positive bacteria were 109 (109.57%) positive from gastric juice samples. The gram negative bacilli Accounted for 78.90% (86/109), Gram-positive cocci accounted for 39.45% (43/109), fungi accounted for 19.27% (21/109), of which mixed infection accounted for 27.52% (30/109). Among Gram-negative bacilli, 72.10% (62/86) were fermentative bacteria, including Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Citrobacter. Non-fermentative bacteria accounted for 27.90% (24/86) , Including Pseudomonas aeruginosa, Acinetobacter spp, Pseudomonas maltophilia. Conclusion: Early bacteriological identification of patients with chronic gastritis is helpful for clinical treatment.