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用溴氰菊酯选育抗性水平中等的河北冀县棉铃虫,经5代4次筛选获得一抗性品系(Del—R),抗性倍数由筛选前F_1代的52.8倍上升到筛选后F_5代的585.9倍(与1983年东台敏感品系相比)。利用此抗性品系进行增效剂(PBo、TPP、DEF)的活体增效作用测定和离体的酶活力研究,无论是对田间品系还是实验室选育的抗性品系,PBo对多功能氧化酶(MFO)的抑制作用是明显的,增效比从3.0—16.5不等,DEF、TPP的增效比分别为2.7和1.0。Del—R品系的多功能氧化酶环氧化活力(AE)是敏感品系的1.5倍,而Del—R品系的酯酶活力是敏感品系的1.01倍,因此多功能氧化酶活力增强是棉铃虫对溴氰菊酯产生抗性的原因之一,酯酶和羧酸酯酶在棉铃虫对溴氰菊酯的抗性中无多大作用。采用剂量对数-死亡机率值(LD—P线)分析法,将棉铃虫对溴氰菊酯的抗性品系和室内培育的敏感品系进行正交、反交和回交试验,得到的抗性遗传研究结果表明,棉铃虫对溴氰菊酯的抗性为常染色体多基因不完全显性遗传。
The delta-cypermethrin-resistant cotton bollworm (Helicoverpa armigera), which was moderate in Hebei Province, was selected to obtain the first-line resistant strain (Del-R) F5 generations of 585.9 times (compared with the 1983 East Taiwan sensitive strains). The synergist (PBo, TPP, DEF) was used to determine the synergistic effect of the synergist (PBo, TPP, DEF) and the enzymatic activity in vitro. The resistance of PBo to multifunctional oxidation The inhibition of enzyme (MFO) was obvious. The efficiency increased from 3.0-16.5. The synergistic ratios of DEF and TPP were 2.7 and 1.0, respectively. The multifunctional oxidase activity (AE) in Del-R strain was 1.5 times that of the susceptible strain, while that in Del-R strain was 1.01 times that of the susceptible strain. Therefore, the enhancement of multifunctional oxidase activity was the highest in Helicoverpa armigera One of the reasons for the resistance of deltamethrin to esterase is that esterase and carboxylesterase have little effect on deltamethrin resistance in cotton bollworm. The logarithmic-death probability (LD-P line) analysis method was used to test the resistance of deltamethrin-resistant strains and susceptible strains cultivated indoors by orthogonal test, anti-test and backcross test. The obtained resistance Genetic studies showed that the resistance of cotton bollworm to deltamethrin was an autosomal dominant gene incompletely dominant.