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目的探讨白细胞介素1α(IL1α)C889T的基因多态性与冠心病的关系。方法根据冠状动脉造影结果将282例患者分为冠心病组(造影结果阳性,126例)和对照组(造影结果阴性,156例),应用聚合酶链反应和限制性内切酶片段长度多态性技术检测两组患者的IL1αC889T基因多态性分布。结果IL1αC889T在该研究人群中的频率分布基因型为CC78.7%,CT20.6%,TT0.7%,等位基因为C502(89.0%),T62(11.0%)。基因型频率符合HardyWeinberg平衡定律。冠心病组CT/TT基因型比对照组有显著性升高(28.6%15.4%,χ2=7.24,P<0.01)。IL1αC889T基因多态性与心肌梗死、冠状动脉病变程度未见明显相关。血清C反应蛋白水平与该位点的基因多态性无关。结论中国人群中存在着IL1αC889T的多态性,T等位基因可能是冠心病的易感基因。
Objective To investigate the relationship between interleukin-1α (IL1α) C889T gene polymorphism and coronary heart disease. Methods According to the results of coronary angiography, 282 patients were divided into coronary heart disease group (126 cases) and control group (negative angiography result, 156 cases). Polymerase chain reaction and restriction fragment length polymorphism Detection of IL1αC889T gene polymorphism in two groups of patients by sex technology. Results The frequency distribution of IL1αC889T in this study population was CC78.7%, CT20.6%, TT0.7%, allele C502 (89.0%) and T62 (11.0%). Genotype frequency in line with HardyWeinberg equilibrium law. The CT / TT genotype in CHD group was significantly higher than that in control group (28.6%, 15.4%, χ2 = 7.24, P <0.01). IL1αC889T gene polymorphism and myocardial infarction, coronary artery lesions no significant correlation. Serum C-reactive protein level and the site of genetic polymorphism has nothing to do. Conclusion The polymorphism of IL1αC889T exists in Chinese population, and T allele may be a susceptibility gene of coronary heart disease.