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目的 :研究激活的多形核白细胞 (PMNs)对血管内皮细胞粘附蛋白 β Catenin和VE Caherin的酪氨酸磷酸化作用。 方法和结果 :采用免疫沉淀和Western印迹法测得激活的PMNs可导致 β Catenin和VE Cadherin酪氨酸磷酸化表达增加 ,酪氨酸激酶抑制剂DAM可显著降低PMNs对内皮细胞的 β Catenin和VE Cadherin的酪氨酸磷酸化水平。 结论 :提示激活的PMNs可能通过 β Catenin和VE Cadherin构型改变来调节内皮细胞屏障功能
AIM: To investigate the tyrosine phosphorylation of activated adhesion molecules β-catenin and VE-Caherin by activated polymorphonuclear leukocytes (PMNs). Methods and Results: Activation of PMNs by immunoprecipitation and Western blotting resulted in increased tyrosine phosphorylation of β Catenin and VE Cadherin, and tyrosine kinase inhibitor DAM significantly decreased β Catenin and VE of endothelial cells Tyrosine phosphorylation level of Cadherin. CONCLUSION: The activated PMNs suggest that endothelial cell barrier function may be regulated by changes of β Catenin and VE Cadherin configurations