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目的通过测量不同发育时期移植物的重量及观察其中生精小管结构和生精细胞组成情况,对去势雄性小鼠中移植的新生小鼠睾丸组织生长和发育进行系统观察。方法将出生1~2d昆明小鼠睾丸移植到7~12周去势雄性免疫缺陷小鼠背部;在移植后不同时间段(分为3d、1~11周和3~6月16个组)取出移植物,计算移植物的回收率,测定移植物重量,观察移植物中生精小管结构及生精细胞的组成。结果从移植的450个新生小鼠睾丸组织,回收到405个移植物,总回收率为90.0%。重量比移植前增加约40倍。移植物中生精小管的发育及各阶段生精细胞出现时间与在正常小鼠中所见基本相同。移植时间超过8周后,生精上皮的退化现象显著增加。结论将新生小鼠睾丸组织异位移植到受体背部后的发育进程与在体情况相同,第1次生精波结束后的时间应为获取精子细胞和精子的最佳时间,大约是移植后5~7周。
OBJECTIVE: To observe the growth and development of testicular tissue in ovariectomized male mice by measuring the weight of the graft at different developmental stages and observing the structure of the seminiferous tubules and the composition of spermatogenic cells. Methods Kunming mouse testes 1 to 2 days after transplantation were transplanted to the back of castrated male immunodeficient mice for 7 to 12 weeks. At different time points after transplantation (divided into 3d, 1 to 11 weeks and 3 to 6 months, 16 groups) The grafts were counted, the recovery of the grafts was calculated, the weight of the grafts was measured, and the structure of seminiferous tubules and the composition of spermatogenic cells in the grafts were observed. Results A total of 405 implants were recovered from 450 newborn mouse testes, with a total recovery of 90.0%. Weight increased about 40 times before transplantation. The development of seminiferous tubules in the graft and the appearance of spermatogenic cells in each stage are basically the same as those seen in normal mice. Degradation of seminiferous epithelium increased significantly after transplantation for more than 8 weeks. CONCLUSIONS: The development of newborn mouse testis after xenotransplantation of the recipient to the back of the recipient is the same as in vivo. The time after the end of the first germinal fluid wave should be the optimal time for obtaining sperm cells and sperm, which is approximately 5 to 7 weeks.