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从崇明大白菜(Brassica pekinensis)上分离到一种病毒,经鉴别寄主测定,在青菜(B.chinensis)、芜菁(B.rapa)上为系统花叶,千红日(Gom-phrema globasa)、苋色藜(Chenopodium amaranticolor)上为局斑,不侵染心叶烟(Nicotiana glutinosa)、黄苗榆(N.tabacum)、黄瓜(Cucumis sa-tivus)。电镜下,病叶组织细胞内观察到风轮状内含体;病叶浸渍液和纯化的病毒制剂中都可见到均一的线状粒体,长660～740nm,宽12.5～15nm。对流兔疫电泳和琼脂扩散试验,病毒与其抗血清产生专一的沉淀线。温度钝化点为55～65℃,稀释终点10~(-3),体外保毒期2～3天。经聚丙烯酰胺凝胶电泳和氨基酸自动分析仪分别测得该病毒的衣壳蛋白亚基分子量为25,000,氨基酸残基数226个。鉴定结果认为它为芜菁花叶病毒崇明分离株(TuMVc.M)。
A virus was isolated from Chinese cabbage Brassica pekinensis, and was identified by the identification host. It was a systemic mosaic on B. chinensis and B. rapa, Gom-phrema globasa, , Spot blight on Chenopodium amaranticolor, non-infecting Nicotiana glutinosa, N. tabacum and cucumber (Cucumis sa-tivus). Electron microscopy, the diseased leaf tissue was observed within the wind wheel-shaped inclusions; diseased leaf immersion fluid and purified virus preparations can be seen in uniform linear mitochondria, long 660 ~ 740nm, width 12.5 ~ 15nm. Converse flow rabbit electrophoresis and agar diffusion test, the virus and its antiserum produced a unique precipitation line. Temperature passivation point of 55 ~ 65 ℃, the end of the dilution 10 ~ (-3), in vitro protection period of 2 to 3 days. The molecular weight of the capsid protein subunit of the virus was 25,000 and the number of amino acid residues was 226 respectively by polyacrylamide gel electrophoresis and amino acid automatic analyzer. Identification results that it is the turnip mosaic Chongming isolates (TuMVc.M).