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目的：观察及评价低聚糖对高强度训练时肠道屏障、氧化应激和炎症标志物的影响。方法64名集训武警战士接受40 d的高强度训练，随机均分为低聚糖组和对照组，低聚糖组每人每日服用低聚糖制剂300 mL。集训前1 d和集训后24 h取样，检测粪便中肠道屏障标志物，检测静脉血中氧化应激和炎症标志物。结果①集训后24 h低聚糖组粪便中的人连蛋白浓度显著低于集训前和对照组（均P<0.05），集训前后α1-抗胰蛋白酶浓度差异无统计学意义。②两组集训前蛋白质羟基化合物（CP）均稍高于正常值。集训后低聚糖组CP降至正常，与集训前和对照组相比均P<0.05。集训40 d后低聚糖组肿瘤坏死因子-α（TNF-α）含量显著低于集训前（P0.05）。结论低聚糖能够促进集训后的肠道屏障功能，降低持续训练造成的氧化应激和炎症反应。“,”Objective To invastigate the effects of oligosaccharides on the markers of intestinal barrier, oxidative stress, and inflammation in intensive training. Methods 64 armed police soldiers undergoing intensive training for 40 days were randomly divided into 2 equal groups:control group following their normal diet and oligosaccharide group receiving 300 mg of oligosaccharides every day in addition. One day before and 24h after the training stool samples were collected to measure the concentrations of zonulin andα1-antitrypsin to estimate the gut leakage. Venous blood samples were collected to determine the concentrations of carbonyl proteins (CP), malondialdehyde (MDA), total oxidation status of lipids (TOS), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6). Results ① The concentration of zonulin in stool after training of the oligosaccharide group was significantly lower than that before training, and significantly lower than that after training of the control group as well (both P<0.05).② The CP concentrations of these groups were both slightly high than normal before training. The CP concentration after training of the oligosaccharide group was significantly lower than that before training (P<0.05), and significantly lower than that after training of the control group as well (P<0.05). The concentration of tumor necrosis factor-α(TNF-α) after training of the oligosaccaride group was significantly lower than that before trainiong (P0.05). Conclusion Oligosaccharides helps improve the intestinal barrier function, and reduce the oxidative stress and inflammatory reaction caused by intensive training.