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目的 :①观察人→小鼠单向混合淋巴细胞反应 ;②建立人→小鼠异种移值物抗宿主病 (XGVHD)模型 ,探讨XGVHD的免疫发生机理。方法 :①无菌采集体小鼠脾淋巴细胞 ,经丝裂霉素处理 ,作刺激细胞 ;取健康成人外周血淋巴细胞 (hPBL) ,作反应细胞 ;异种单向混合淋巴细胞培养(one wayXMLC) 1周 ;②摧毁受者 (小鼠 )免疫系统 ;将hPBL经尾静脉注入小鼠体内 ,两周后检测脾、肾组织。结果 :①小鼠脾淋巴细胞明显刺激人外周血淋巴细胞 (hPBL)增殖 ,3 H TdR掺入值 (cpm)显著增高 (P <0 0 5 ) ;CD4 ,CD8,IgG ,IgM细胞含量明显升高 ,有显著差异 (P <0 0 5 )。②实验组 (注射hPBL)动物诱发XGVHD ,脾、肾组织中发现人中CD4、CD8、IgG、IgM细胞 ,有Fas蛋白表达和少量调亡细胞出现。结论 :①人→小鼠单向混合淋巴细胞培养 (one wayXMLC) ,具有明显细胞增殖反应 ;②本实验方法成功诱发XGVHD ,建立人→小鼠异种移殖物抗宿主 (XGVHD)模型
Objectives: ① To observe the human → mouse unidirectional mixed lymphocyte reaction; ② To establish a human → mouse xenograft versus host disease (XGVHD) model to investigate the mechanism of XGVHD immunity. Methods: (1) Spleen lymphocytes from mice were collected aseptically and treated with mitomycin C to stimulate cells. Healthy adult peripheral blood lymphocytes (hPBL) were used as reaction cells. One way XMLC (mixed lymphocyte culture) 1 week; ② destroy the recipient (mouse) immune system; hPBL was injected through the tail vein into the body of mice, two weeks after the detection of spleen and kidney tissue. Results: ① The splenic lymphocytes of mice significantly stimulated the proliferation of human peripheral blood lymphocytes (hPBL), and the incorporation of 3 H TdR (cpm) was significantly increased (P <0 05). The contents of CD4, CD8, IgG and IgM were significantly increased High, there are significant differences (P <0 05). ② In experimental group (injected with hPBL), XGVHD, spleen and kidney were found in human CD4, CD8, IgG, IgM cells, Fas protein expression and a small amount of apoptotic cells appeared. Conclusions: ① Human one-way mixed lymphocyte culture (one wayXMLC) in mice has obvious cell proliferative response. ② The XGVHD was successfully induced in this experiment and the human-mouse xenograft versus host (XGVHD) model was established