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本文介绍用少量血样品以Normal缓冲溶液为介质在4℃过夜保温条件下红细胞胰岛素受体测定方法。红细胞胰岛素受体与~(125)I-胰岛素结合的竞争抑制曲线和Scatchard作图性质与文献报导结果相同。对人、家兔、狗和大鼠四种不同种属的红细胞胰岛素受体测定最大结合率(B/T％)在10—15％范围。非专一性结合平均为3.5％左右。用Normal缓冲溶液代替BufferG作保温介质同样能得到较好结果。四种不同种属红细胞胰岛素受体数目有差异,依次是狗>兔>大鼠>人。低温(4℃)条件下结合试验可减少胰岛素-受体复合物的降解速度。本方法适合于临床胰岛素受体测定。
This article describes a small amount of blood samples to Normal buffer solution as a medium at 4 ℃ overnight incubation conditions erythrocyte insulin receptor assay. Competitive inhibition and Scatchard mapping of the binding of erythrocyte insulin receptor to ~ (125) I-insulin are the same as reported in the literature. The maximum binding rate (B / T%) for erythrocyte insulin receptors measured in four different species of human, rabbit, dog and rat was in the range of 10-15%. Non-specific combination of an average of about 3.5%. Normal buffer solution instead of BufferG for insulation medium can also get better results. There were differences in the numbers of erythrocyte insulin receptors in four different species, followed by dog> rabbit> rat> human. Binding assays at low (4 ° C) conditions reduce the rate of degradation of the insulin-receptor complex. This method is suitable for clinical insulin receptor assay.