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目的:建立高效液相色谱法测定跌打片中人参皂苷Rg★和人参皂苷Rb★的含量测定方法。方法:采用Luna C_(18)色谱柱(250 mm×4.6 mm,5μm);柱温:35℃;流动相为乙腈-水,梯度洗脱;流速1.0 ml·min~(-1);检测波长203 nm。结果:人参皂苷Rg★线性范围为0.340～5.094μg(r=1.000 0),人参皂苷Rb★线性范围为0.303～0.454μg(r=1.000 0);平均加样回收率:人参皂苷Rg_1★为97.17%,RSD=1.09%(n=6),人参皂苷Rb★为102.63%,RSD=1.18%(n=6)。结论:该方法简便可靠,可用于跌打片的质量控制。
OBJECTIVE: To establish a HPLC method for the determination of ginsenoside Rg and ginsenoside Rb in tablets. METHODS: The mobile phase consisted of acetonitrile-water gradient elution with Luna C 18 column (250 mm × 4.6 mm, 5 μm) at a column temperature of 35 ℃. The flow rate was 1.0 ml · min -1. The detection wavelength 203 nm. Results: The linear range of ginsenoside Rg ★ was 0.340 ~ 5.094μg (r = 1.000 0). The linear range of ginsenoside Rb ★ was 0.303 ~ 0.454μg (r = 1.000 0). The average recovery rate of ginsenoside Rg_1 ★ was 97.17 %, RSD = 1.09% (n = 6), ginsenoside Rb ★ 102.63%, RSD = 1.18% (n = 6). Conclusion: This method is simple and reliable and can be used for the quality control of beetles.