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用ＨＢＶ ＤＮＡ二聚体转染高度分化的人肝癌细胞株（ＨｅｐＧ２），筛选出具有分泌病毒抗原、核酸以及释放４２ｎｍ Ｄａｎｅ颗粒能力的ｚ７－４细胞株。本实验结果支持３．５ＫｂｍＲＮＡ是前病毒基因，以它作模板可逆转录成１．６Ｋｂ（－）ｓｓＤＮＡ，再合成（＋）ｓｓＤＮＡ，最后装配成４．０Ｋｂ和３．２ＫｂｄｓＤＮＡ。在ＤＮＡ印迹法中位于４．０Ｋｂ（Ｄ１）和３．２Ｋｂ（Ｄ２）处是病毒的松驰环状双股ＤＮＡ（ＲＣＤＮＡ），而１．６Ｋｂ和１．２Ｋｂ处的２条ＤＮＡ带（Ｓ１和Ｓ２）均为单股ＤＮＡ，这些不同位置的ＤＮＡ带与病毒复制过程中ＤＮＡ的构型和片断大小不同有关。本实验未能发现２．０ＫｂｃｃｃＤＮＡ，但提示了负股ＤＮＡ５’端连接有蛋白质引物。
The highly differentiated human hepatoma cell line (HepG2) was transfected with HBV DNA dimer and the z7-4 cell line was screened for its ability to secrete viral antigens, nucleic acids and release 42 nm Dane particles. Our results support the fact that the 3.5 kb mRNA is a proviral gene, which can be used as a template for reversible transcription into 1.6 kb (-) ssDNA, (+) ssDNA and finally 4.0 kb and 3.2 kb dsDNA. Lyocell double stranded DNA (RCDNA), located at 4.0 Kb (D1) and 3.2 Kb (D2) in the Southern blot, while the two DNA strands at 1.6 Kb and 1.2 Kb (S1 And S2) are single-stranded DNA. The DNA bands in these different locations are related to the DNA configuration and the size of the fragments during virus replication. This experiment failed to find 2.0KbcccDNA, but suggested that negative strand DNA 5 ’end connected with a protein primer.