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目的:探讨载药纳米粒联合光动力治疗应用于大鼠卵巢癌腹水瘤模型治疗的疗效。方法:制备包裹竹红菌乙素(HB)的聚氰基丙烯酸丁酯纳米粒(PBCA-NP)及建立Fischer 344大鼠卵巢癌腹水瘤模型。大鼠腹腔注射HB的DMSO溶液及HB-PBCA-NP溶液,观察不同药物剂型在大鼠体内分布,得到竹红菌乙素及竹红菌乙素纳米粒的药物动力学参数。将荷瘤大鼠随机分成4组:对照组未给予任何处理,手术组只接受手术治疗,实验组分别给予HB溶液及其纳米粒溶液,并接受手术及光动力治疗,观察生存时间。结果:纳米粒稳定,可用于体内实验。血清及肿瘤组织中,纳米粒组药物浓度达高峰的时间明显延迟。手术联合光动力治疗中位生存时间(95天和99天)比单纯手术(84天)长,可延长生存时间(P<0.05)。结论:纳米粒可使药物在体内达到缓释,手术联合光动力治疗可延长生存时间。
Objective: To investigate the efficacy of drug-loaded nanoparticles combined with photodynamic therapy in the treatment of rat ovarian cancer ascites tumor model. Methods: Polybutylcyanoacrylate nanoparticles (PBCA-NP) encapsulated with hypocrellin B (HB) and the model of Fischer 344 rat ovarian ascites tumor were established. HBsAg and HB-PBCA-NP solution were injected intraperitoneally into rats. The pharmacokinetics parameters of different formulations of hypocrellin B and hypocrellin B nanoparticles were observed. The tumor-bearing rats were randomly divided into 4 groups: the control group did not receive any treatment, the surgical group received only surgical treatment, the experimental group were given HB solution and its nanoparticle solution, and underwent surgery and photodynamic therapy to observe the survival time. Results: Nanoparticles are stable and can be used in in vivo experiments. Serum and tumor tissue, the nanoparticle drug concentration peaked significantly delayed the time. The median survival time (95 days and 99 days) after surgery combined with photodynamic therapy was longer than pure surgery (84 days), prolonging the survival time (P <0.05). CONCLUSION: Nanoparticles can prolong the drug release in vivo and the combination of surgery and photodynamic therapy can prolong the survival time.