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本文~3H-TdR参入细胞DNA为指标研究了EGF等生长调节因子对小鼠腹水癌细胞DNA合成的影响,发现不同癌细胞对EGF等生长因子的敏感性有所差异,考虑到这也许与肿瘤细胞自身特性如恶性度有关。为了进一步探讨恶性度与这一敏感性是否相关,我们观察并比较了C_3H10T1/2CL_8(一种来源于鼠胚的正常成纤维细胞,简称NC_3H_(10)及转化的C_3H_(10)T1/2CL_8(用~3H-TdR转化的上述细胞,简称TC_3H_(10))对EGF等生长因子的敏感性。实验证明,细胞恶性转化后,对EGF的敏感性明显降低,~3H-TdR参入率降至原先的1/4以下。用DBcAMP作用于NC_3H_(10)和TC_3H_(10)均能抑制~3H-TdR参入DNA并可抑制EGF诱导的~3H-TdR参入作用。因此,我们认为,有关物理的致癌因素如放射性同位素,像生物、化学的致癌因素一样,亦能引起其转化细胞对外源性生长调节因子敏感性的改变。
In this paper, ~3H-TdR incorporation into cell DNA was used as an index to study the effect of EGF and other growth regulators on DNA synthesis in mouse ascites carcinoma cells. It was found that the sensitivity of different cancer cells to EGF and other growth factors is different, considering that this may be related to tumors. The characteristics of the cells themselves are related to the degree of malignancy. To further investigate whether the degree of malignancy is related to this sensitivity, we observed and compared C_3H10T1/2CL_8 (a normal fibroblast derived from mouse embryos, referred to as NC_3H_(10) and transformed C_3H_(10)T1/2CL_8 ( The above cells transformed with ~3H-TdR, abbreviated as TC_3H_(10), are sensitive to EGF and other growth factors. Experiments have shown that after malignant transformation, the sensitivity to EGF is significantly reduced, and the rate of ~3H-TdR incorporation is reduced to the original level. 1/4 or less of the effect of DBcAMP on NC_3H_(10) and TC_3H_(10) inhibits ~3H-TdR incorporation into DNA and inhibits EGF-induced ~3H-TdR incorporation. Therefore, we believe that the related physical carcinogenesis Factors such as radioactive isotopes, like biological and chemical carcinogenic factors, can also cause changes in the sensitivity of their transformed cells to exogenous growth regulators.